Nicolau Renata Amadei, Martinez Manel Santafé, Rigau Josepa, Tomàs Josep
Institute for Research and Development, Universidade do Vale do Paralba (Univap)-SJC, Brazil.
Lasers Surg Med. 2004;34(3):277-84. doi: 10.1002/lsm.20006.
Low level laser therapy (LLLT) in specific wavelengths and fluence maintains the electrophysiological activity of injured peripheral nerve in rats, preventing scar formation (at injury site) as well as degenerative changes in the corresponding motor neurons of the spinal cord, thus accelerating regeneration of the injured nerve. We studied the effect of LLLT on the neurotransmitter release in neuromuscular junctions of the mouse diaphragm.
STUDY DESIGN/MATERIALS AND METHODS: Thirty-nine diaphragm muscles were studied. LLLT with GaAlAs 655 nm (1-12 J/cm(2)) was used. Neurotransmitter release was studied by conventional intracellular recording techniques on curarised or high magnesium media. Quantal content, amplitude, latency and rise time were analysed for end-plate potentials (EPPs). Frequency and amplitude were evaluated for the miniature end-plate potentials (MEPPs). Short-term plasticity of the neurotransmitter release (fast facilitation) was also evaluated by paired pulse stimulation.
This study showed that LLLT (655 nm) in these doses has no detectable physiological effect on the motor end-plate neurotransmitter release in mice.
特定波长和能量密度的低强度激光疗法（LLLT）可维持大鼠受损周围神经的电生理活性，防止（损伤部位）瘢痕形成以及脊髓相应运动神经元的退行性变化，从而加速受损神经的再生。我们研究了LLLT对小鼠膈肌神经肌肉接头处神经递质释放的影响。
研究设计/材料与方法：对39块膈肌进行了研究。使用波长为655 nm的GaAlAs进行LLLT（能量密度为1 - 12 J/cm²）。通过在箭毒化或高镁培养基上的传统细胞内记录技术研究神经递质释放。分析终板电位（EPPs）的量子含量、幅度、潜伏期和上升时间。评估微小终板电位（MEPPs）的频率和幅度。还通过配对脉冲刺激评估神经递质释放的短期可塑性（快速易化）。
本研究表明，这些剂量的LLLT（655 nm）对小鼠运动终板神经递质释放没有可检测到的生理影响。
