曼谷阿萨亚菌新种，一种属于α-变形菌纲的醋酸菌。
Asaia krungthepensis sp. nov., an acetic acid bacterium in the alpha-Proteobacteria.
作者信息
Yukphan Pattaraporn, Potacharoen Wanchern, Tanasupawat Somboon, Tanticharoen Morakot, Yamada Yuzo
机构信息
BIOTEC Culture Collection, BIOTEC Central Research Unit, National Centre for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand.
Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Pathumwan, Bangkok 10330, Thailand.
出版信息
Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):313-316. doi: 10.1099/ijs.0.02734-0.
Three bacterial strains were isolated from flowers collected in Bangkok, Thailand, by an enrichment-culture approach for acetic acid bacteria. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were located in the lineage of the genus Asaia but constituted a cluster separate from the type strains of Asaia bogorensis and Asaia siamensis. The DNA base composition of the isolates was 60.2-60.5 mol% G+C, with a range of 0.3 mol%. The isolates constituted a taxon separate from Asaia bogorensis and Asaia siamensis on the basis of DNA-DNA relatedness. The isolates had morphological, physiological, biochemical and chemotaxonomic characteristics similar to those of the type strains of Asaia bogorensis and Asaia siamensis, but the isolates grew on maltose. The major ubiquinone was Q(10). On the basis of the results obtained, the name Asaia krungthepensis sp. nov. is proposed for the isolates. The type strain is isolate AA08(T) (=BCC 12978(T)=TISTR 1524(T)=NBRC 100057(T)=NRIC 0535(T)), which had a DNA G+C content of 60.3 mol% and was isolated from a heliconia flower ('paksaasawan' in Thai; Heliconia sp.) collected in Bangkok, Thailand.
通过醋酸菌富集培养法，从泰国曼谷采集的花卉中分离出三株细菌菌株。基于16S rRNA基因序列的系统发育分析表明，这些分离株位于阿萨亚属的谱系中，但构成了一个与博戈里亚阿萨亚菌和暹罗阿萨亚菌模式菌株不同的簇。分离株的DNA碱基组成为60.2 - 60.5 mol% G+C，范围为0.3 mol%。基于DNA - DNA相关性，分离株构成了一个与博戈里亚阿萨亚菌和暹罗阿萨亚菌不同的分类单元。分离株具有与博戈里亚阿萨亚菌和暹罗阿萨亚菌模式菌株相似的形态、生理、生化和化学分类特征，但分离株能在麦芽糖上生长。主要泛醌为Q(10)。根据所获得的结果，为这些分离株提议命名为曼谷阿萨亚菌（Asaia krungthepensis）新种。模式菌株为分离株AA08(T)（=BCC 12978(T)=TISTR 1524(T)=NBRC 100057(T)=NRIC 0535(T)），其DNA G+C含量为60.3 mol%，从泰国曼谷采集的一种蝎尾蕉属花卉（泰语为“paksaasawan”；Heliconia sp.）中分离得到。
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