Taxonomic characterization and antimicrobial compound production from BDK01 isolated from marine sediment.

sp. isolated from marine sediment collected from Palk Strait, Bay of Bengal was investigated for its antagonistic potential. The isolate exhibited antimicrobial activity against selected bacterial strains of clinical importance such as MTCC 3160, NCIM 2327, (methicillin resistant), MTCC 1698, (ESBL), MTCC 1457, and Phenotypic and molecular characterization ascertained the isolate BDK01 as . Media optimization with one variable-at-a-time strategy was attempted to identify the ideal concentrations of starch (5-15 g/l), casein (0.01-0.05 g/l), NaCl 1.0-3.0 g/l, pH (4.0-9.0 g/l), temperature (25-45 °C) and inoculum level (0.5-5 ml) towards achieving maximum antimicrobial compound production. Statistical optimization of production media was carried by establishing an 11 variables 17 run experiment through PB model which evinced starch, calcium carbonate, pH and inoculum concentration that highly influenced bioactive compound production. Spectral data of active ethyl acetate extract revealed the presence of various bioactive compounds such as Salicyl alcohol, -phenyl benzamide. 6-Octadecenoic acid, (), 1,3,5-Cycloheptatriene. Antiproliferation activity of active fraction against MCF-7 Cell line exhibited IC value of 9.5 µg/ml. Overall, it is observed that the marine actinomycete BDK01 could be employable as promising strain for novel antimicrobial and cytotoxic metabolites.