Vancanneyt M, Mengaud J, Cleenwerck I, Vanhonacker K, Hoste B, Dawyndt P, Degivry M C, Ringuet D, Janssens D, Swings J
BCCM/LMG Bacteria Collection, Ghent University, Ghent, Belgium.
DANONE Vitapole, Vitavaleur, Palaiseau, France.
Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):551-556. doi: 10.1099/ijs.0.02912-0.
Fourteen homofermentative lactic acid bacteria that were isolated from kefir grains and kefir fermented milks were assigned to either Lactobacillus kefiranofaciens or Lactobacillus kefirgranum, based on their characteristic morphotypes, phenotypic features and SDS-PAGE profiles of whole-cell proteins. Further genotypic analyses on representative strains from both taxa demonstrated that L. kefiranofaciens and L. kefirgranum share 100 % 16S rDNA sequence similarity and belong phylogenetically to the Lactobacillus acidophilus species group. DNA-DNA binding values of >79 % and analogous DNA G+C contents of 37-38 mol% showed that the strains studied belonged to one species: L. kefirgranum is a later synonym of L. kefiranofaciens. An emended description is proposed for L. kefiranofaciens. Due to the specific morphological and biochemical characteristics of these taxa in kefir grain formation, it is proposed that L. kefirgranum should be reclassified as L. kefiranofaciens subsp. kefirgranum subsp. nov.
从开菲尔粒和开菲尔发酵乳中分离出的14株同型发酵乳酸菌，根据其特征形态型、表型特征和全细胞蛋白的SDS-PAGE图谱，被归类为开菲尔糖乳杆菌（Lactobacillus kefiranofaciens）或开菲尔粒乳杆菌（Lactobacillus kefirgranum）。对这两个分类单元的代表性菌株进行的进一步基因分析表明，开菲尔糖乳杆菌和开菲尔粒乳杆菌的16S rDNA序列相似性为100%，在系统发育上属于嗜酸乳杆菌种组。>79%的DNA-DNA结合值和37-38 mol%的类似DNA G+C含量表明，所研究的菌株属于同一个种：开菲尔粒乳杆菌是开菲尔糖乳杆菌的后选同物异名。本文提出了对开菲尔糖乳杆菌的修订描述。由于这些分类单元在开菲尔粒形成过程中具有特定的形态和生化特征，建议将开菲尔粒乳杆菌重新分类为开菲尔糖乳杆菌开菲尔粒亚种（L. kefiranofaciens subsp. kefirgranum subsp. nov.）。
