Iomini Carlo, Tejada Karla, Mo Wenjun, Vaananen Heikki, Piperno Gianni
Center for Anatomy and Functional Morphology, Mount Sinai School of Medicine, 1 Gustave L. Levy Pl., Box 1007, New York, NY 10029, USA.
J Cell Biol. 2004 Mar 15;164(6):811-7. doi: 10.1083/jcb.200312133.
We identified primary cilia and centrosomes in cultured human umbilical vein endothelial cells (HUVEC) by antibodies to acetyl-alpha-tubulin and capillary morphogenesis gene-1 product (CMG-1), a human homologue of the intraflagellar transport (IFT) protein IFT-71 in Chlamydomonas. CMG-1 was present in particles along primary cilia of HUVEC at interphase and around the oldest basal body/centriole at interphase and mitosis. To study the response of primary cilia and centrosomes to mechanical stimuli, we exposed cultured HUVEC to laminar shear stress (LSS). Under LSS, all primary cilia disassembled, and centrosomes were deprived of CMG-1. We conclude that the exposure to LSS ends the IFT in cultured endothelial cells.
我们通过抗乙酰化α-微管蛋白抗体和毛细管形态发生基因-1产物（CMG-1，衣藻中鞭毛内运输（IFT）蛋白IFT-71的人类同源物），在培养的人脐静脉内皮细胞（HUVEC）中鉴定出初级纤毛和中心体。CMG-1在间期存在于HUVEC初级纤毛周围的颗粒中，在间期和有丝分裂时存在于最老的基体/中心粒周围。为了研究初级纤毛和中心体对机械刺激的反应，我们将培养的HUVEC暴露于层流切应力（LSS）。在LSS作用下，所有初级纤毛解体，中心体失去CMG-1。我们得出结论，暴露于LSS会终止培养的内皮细胞中的IFT。
