Mei Ying, Beers Kathryn L, Byrd H C Michelle, VanderHart David L, Washburn Newell R
Polymers Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA.
J Am Chem Soc. 2004 Mar 24;126(11):3472-6. doi: 10.1021/ja039583d.
A versatile methodology to prepare hybrid biomaterials by atom transfer radical polymerization from resin-supported peptides has been established. As an example, we have synthesized a GRGDS-functionalized poly(2-hydroxyethyl methacrylate). The peptide-polymer was characterized by solid-state (13)C NMR and GPC and found to have a number average molecular weight of 4420 and a polydispersity of 1.47. These values are comparable to those obtained from solution-phase syntheses, suggesting the ATRP reaction is successful from a peptide-conjugated solid support. Solid-state (13)C NMR was used to characterize multiple steps in the reaction, and the synthesis was found to be near quantitative. We have performed cell adhesion experiments and observed the GRGDS sequence-promoted cell adhesion, whereas unfunctionalized poly(2-hydroxyethyl methacrylate) did not. By incorporating cell-signaling moieties in materials with defined molecular architecture, it will be possible to control the interactions between polymeric materials and biological systems.
已经建立了一种通过原子转移自由基聚合从树脂支持的肽制备杂化生物材料的通用方法。例如，我们合成了一种GRGDS功能化的聚甲基丙烯酸2-羟乙酯。通过固态（13）C NMR和凝胶渗透色谱对肽聚合物进行了表征，发现其数均分子量为4420，多分散性为1.47。这些值与从溶液相合成获得的值相当，这表明从肽共轭固体支持物进行的原子转移自由基聚合反应是成功的。固态（13）C NMR用于表征反应中的多个步骤，发现合成接近定量。我们进行了细胞粘附实验，观察到GRGDS序列促进了细胞粘附，而未功能化的聚甲基丙烯酸2-羟乙酯则没有。通过将细胞信号部分掺入具有确定分子结构的材料中，将有可能控制聚合物材料与生物系统之间的相互作用。
