Boyce John D, Wilkie Ian, Harper Marina, Paustian Mike L, Kapur Vivek, Adler Ben
Veterinary Pathology and Anatomy, School of Veterinary Science and Animal Production, The University of Queensland, Qld 4072, Australia.
Microbes Infect. 2004 Mar;6(3):290-8. doi: 10.1016/j.micinf.2003.12.004.
We have recently reported the gene expression profile of Pasteurella multocida during growth in the blood of chickens with fowl cholera. Here we report the gene expression profile of P. multocida during growth in the livers of similarly infected chickens. We compared expression profiles of bacteria harvested from the livers of infected chickens with late-stage fowl cholera with those of bacteria grown in rich medium. Independent analysis of bacterial expression profiles from three individual chickens indicated that 93 P. multocida genes were always differentially expressed during growth in liver tissue. Of these 93 genes, 49 were upregulated and 44 downregulated in the host. Many of the upregulated genes were involved in energy production and conversion (9/49) and carbohydrate transport and metabolism (8/49), and a number of these have been shown to be induced under anaerobic conditions in other species. The downregulated genes were generally of unknown or poorly characterised functions (14/44). Comparison of the differentially regulated gene sets identified for growth in liver with those identified previously for growth in blood allowed the identification of a core set of 13 upregulated and 16 downregulated genes that were differentially regulated in at least five of the six infections studied.
我们最近报道了多杀性巴氏杆菌在患有禽霍乱的鸡血液中生长期间的基因表达谱。在此，我们报道多杀性巴氏杆菌在同样感染的鸡肝脏中生长期间的基因表达谱。我们将从患有晚期禽霍乱的感染鸡肝脏中收获的细菌的表达谱与在丰富培养基中生长的细菌的表达谱进行了比较。对来自三只个体鸡的细菌表达谱进行的独立分析表明，93个多杀性巴氏杆菌基因在肝脏组织生长期间总是差异表达。在这93个基因中，49个在宿主体内上调，44个下调。许多上调的基因参与能量产生和转换（49个中的9个）以及碳水化合物运输和代谢（49个中的8个），其中一些已被证明在其他物种的厌氧条件下被诱导。下调的基因通常功能未知或特征不明确（44个中的14个）。将在肝脏中生长所鉴定的差异调节基因集与先前在血液中生长所鉴定的基因集进行比较，使得能够鉴定出一组核心基因，其中13个上调基因和16个下调基因在至少六项研究的感染中的五项中差异调节。
