State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FEMS Microbiol Lett. 2012 Jun;331(2):105-12. doi: 10.1111/j.1574-6968.2012.02559.x. Epub 2012 Apr 17.
Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera in poultry, hemorrhagic septicemia in cattle, atropic rhinitis in swine, and snuffles in rabbits. The differentially expressed gene profile of P. multocida in infected rabbit livers was identified and compared with that from in vitro culture by selective capture of transcribed sequences. A total of 31 genes were identified, of which 28 encoded enzymes for amino acid biosynthesis and metabolism, intermediary metabolism, and energy metabolism, or proteins for regulatory adaptive responses, general microbial stress response, transport proteins, and secreted proteinases. Three were unknown, novel genes. Five genes representing different categories were chosen randomly and verified by real-time reverse transcriptase-polymerase chain reaction analysis. All were upregulated by P. multocida in infected rabbit livers, with changes ranging from 1.61- to 13.55-fold when compared with in vitro cultures. This study has identified genes of P. multocida that are upregulated during infection of rabbit livers when compared with in vitro growth conditions. The genes will provide a molecular basis for further study of the pathogenesis of P. multocida.
多杀巴斯德菌，一种革兰氏阴性无动力的球杆菌，是家禽霍乱、牛出血性败血症、猪萎缩性鼻炎和兔传染性鼻炎的病原体。通过选择转录序列捕获，鉴定和比较了感染兔肝脏中的多杀巴斯德菌与体外培养的差异表达基因谱。共鉴定出 31 个基因，其中 28 个编码用于氨基酸生物合成和代谢、中间代谢和能量代谢的酶，或用于调节适应性反应、一般微生物应激反应、转运蛋白和分泌蛋白酶的蛋白质。其中三个是未知的、新的基因。随机选择了代表不同类别的五个基因，并通过实时逆转录聚合酶链反应分析进行了验证。与体外培养相比，所有基因在感染兔肝脏中的表达均上调，变化范围从 1.61 倍至 13.55 倍。本研究鉴定了感染兔肝脏中与体外生长条件相比上调的多杀巴斯德菌基因。这些基因将为进一步研究多杀巴斯德菌的发病机制提供分子基础。
