Ku S K, Lee H S, Lee J H
Department of Biology, Faculty of Basic Science, Kyungsan Univesrity, Kyungsan, Kyungpook, Republic of Korea.
Anat Histol Embryol. 2004 Feb;33(1):42-8. doi: 10.1111/j.1439-0264.2004.00508.x.
The distributions and frequencies of some endocrine cells in the eight portions of the gastrointestinal tract (GIT) of BALB/c mouse were studied. Endocrine cells were stained using immunohistochemical method with seven types of antisera against bovine chromogranin (BCG), serotonin, gastrin, cholecystokinin (CCK)-8, somatostatin, glucagon and human pancreatic polypeptide (HPP), and the regional distributions and their relative frequencies were observed in the eight portions of the GIT of BALB/c mice. All seven types of immunoreactive (IR) cells were identified. Most of the IR cells in the intestinal portion were generally spherical or spindle in shape (open type cell) while round-shaped cells (closed type cell) were found in the intestinal gland and stomach regions occasionally. Their relative frequencies varied according to each portion of the GIT. BCG-IR cells were observed throughout the whole GIT except for the rectum and they were most predominant in the pylorus. Serotonin-IR cells were detected throughout the whole GIT and they showed the highest frequency in the fundus. Gastrin- and CCK-IR cells were restricted to the pylorus and duodenum with a majority in the pylorus and rare or a few frequencies in the duodenum. Compared with other mammals, somatostatin-IR cells were restricted to the fundus and pylorus with a few frequencies, respectively. In addition, glucagon- and HPP-IR cells were restricted to the fundus and duodenum, respectively, with relative low frequencies. Some species-dependent unique distributions and frequencies of endocrine cells were observed in the GIT of BALB/c mouse compared with other rodents.
研究了BALB/c小鼠胃肠道（GIT）八个部分中一些内分泌细胞的分布和频率。使用针对牛嗜铬粒蛋白（BCG）、血清素、胃泌素、胆囊收缩素（CCK）-8、生长抑素、胰高血糖素和人胰多肽（HPP）的七种抗血清，通过免疫组织化学方法对内分泌细胞进行染色，并观察了BALB/c小鼠GIT八个部分中的区域分布及其相对频率。鉴定出了所有七种免疫反应性（IR）细胞。肠道部分的大多数IR细胞通常呈球形或纺锤形（开放型细胞），而圆形细胞（封闭型细胞）偶尔出现在肠腺和胃区域。它们的相对频率因GIT的每个部分而异。除直肠外，在整个GIT中均观察到BCG-IR细胞，且在幽门处最为丰富。在整个GIT中均检测到血清素-IR细胞，且在胃底频率最高。胃泌素和CCK-IR细胞局限于幽门和十二指肠，多数在幽门，在十二指肠中频率罕见或较低。与其他哺乳动物相比，生长抑素-IR细胞分别局限于胃底和幽门，频率较低。此外，胰高血糖素-IR细胞和HPP-IR细胞分别局限于胃底和十二指肠，频率相对较低。与其他啮齿动物相比，在BALB/c小鼠的GIT中观察到了一些物种依赖性的内分泌细胞独特分布和频率。
