Park Yong Ho, Joo Yi Seok, Park Joo Youn, Moon Jin San, Kim So Hyun, Kwon Nam Hoon, Ahn Jong Sam, Davis William C, Davies Christopher J
Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
J Vet Sci. 2004 Mar;5(1):29-39.
Bovine mastitis is an infectious disease with a major economic influence on the dairy industry worldwide. Many factors such as environment, pathogen, and host affect susceptibility or resistance of an individual cow to bovine mastitis. Recently, there has been considerable interest in defining genetic and immunological markers that could be used to select for improved disease resistance. In this study we have analyzed the lymphocyte subpopulations of mastitis-resistant and susceptible cows using monoclonal antibodies specific for bovine leukocyte differentiation antigens and flow cytometry. We have also used a microarray typing technique to define the bovine leukocyte antigen (BoLA) class I and class II haplotypes associated with resistance or susceptibility to bovine mastitis. A striking finding of the present study is that susceptibility to mastitis was associated with major histocompatibility complex (MHC) haplotypes that have only a single set of DQ genes. The study also revealed that susceptible cows had CD4:CD8 ratios of less than one in both their mammary gland secretions and peripheral blood. These results raise the possibility that the number of DQ genes that a cow has and/or a cow's CD4:CD8 ratio could be used as indicators of susceptibility to bovine mastitis.
牛乳腺炎是一种对全球乳制品行业有重大经济影响的传染病。环境、病原体和宿主等许多因素会影响个体奶牛对牛乳腺炎的易感性或抵抗力。最近，人们对确定可用于选择提高抗病能力的遗传和免疫标记物产生了浓厚兴趣。在本研究中，我们使用针对牛白细胞分化抗原的单克隆抗体和流式细胞术分析了抗乳腺炎和易患乳腺炎奶牛的淋巴细胞亚群。我们还使用微阵列分型技术来确定与牛乳腺炎抗性或易感性相关的牛白细胞抗原（BoLA）I类和II类单倍型。本研究的一个显著发现是，乳腺炎易感性与仅具有一组DQ基因的主要组织相容性复合体（MHC）单倍型相关。该研究还表明，易患乳腺炎的奶牛在其乳腺分泌物和外周血中的CD4:CD8比率均小于1。这些结果增加了这样一种可能性，即奶牛拥有的DQ基因数量和/或奶牛的CD4:CD8比率可作为牛乳腺炎易感性的指标。
