Perevoshchikov A G, Bukhvalov I B, Mikhaĭlov E A
Biull Eksp Biol Med. 1978;86(7):58-61.
Ultracytochemical investigation of ATP-ase activity was carried out in parietal cells of the mucosa and in cancer cells of human stomach carcinoma possessing a similar ultrastructure. In parietal cells the reaction product of ATP-ase was observed on the membranes of microvilli of intracellular canaliculi, on the membranes delineating the lateral intercellular space, on the basal plasmolemma and in the nucleoli. The reaction product was absent on the membranes of tubuvesicles and on the apical surface of the plasmalemma. In cancer cells the reaction product was found on the membranes of the microvilli of the intracellular canaliculi, basal plasmolemma and in the nucleoli. Comparative examination of ATP-ase activity in these cells implies that at least the part of the mechanism of hydrochloric acid secretion which is involved in the transfer of H+ and Cl- is retained in cancer cells. A steady decrease in hydrochlorid acid secretion observed in the stomach mucosa in cancer as well as in the tumour itself seems to be associated with other mechanisms.
对胃黏膜壁细胞以及具有相似超微结构的人胃癌癌细胞进行了ATP酶活性的超微细胞化学研究。在壁细胞中，ATP酶的反应产物出现在细胞内小管微绒毛的膜上、界定细胞间侧间隙的膜上、基底质膜上以及核仁中。在管泡的膜和质膜的顶端表面没有反应产物。在癌细胞中，反应产物出现在细胞内小管微绒毛的膜、基底质膜上以及核仁中。对这些细胞中ATP酶活性的比较研究表明，至少参与H⁺和Cl⁻转运的盐酸分泌机制的一部分在癌细胞中得以保留。在胃癌患者的胃黏膜以及肿瘤本身中观察到的盐酸分泌持续减少似乎与其他机制有关。
