Koopman D G, Kezić S, Verberk M M
Coronel Institute for Occupational and Environmental Health, Academic Medical Centre, University of Amsterdam, the Netherlands.
Br J Dermatol. 2004 Mar;150(3):493-9. doi: 10.1046/j.1365-2133.2004.05765.x.
Little is known of the predictive value of methods to test an individual's susceptibility to acquiring occupational contact dermatitis. Recently, the recovery rate after induced irritation was suggested for this purpose. Although it is likely that repeated exposure to sodium lauryl sulphate (SLS) is preferable to a single application, there is little evidence to support this idea. Similarly, little is known about whether the outcome of a repeated SLS test can be predicted by a brief test.
We studied the relationship between the skin reaction after a repeated SLS test and two brief tests, devoting special attention to the recovery rate.
In 29 healthy volunteers, we measured transepidermal water loss (TEWL) and erythema after applying 0.03, 0.1 and 0.3% SLS for 6 h, 3 days per week, over a course of 3 weeks. The data were compared with the effects after applying 0.1, 0.3 and 1.0% SLS for 24 h and with 10 and 15 repetitions of tape stripping.
A poor correlation was found between the repeated test and the brief SLS test, or tape stripping, when using an increase in TEWL (r = 0.04 and 0.26, respectively) or its recovery rate (r = - 0.01 and 0.42, respectively).
We presume that in a repeated test of sufficient duration, additional mechanisms come into play that are absent in a brief test, e.g. an alteration in the thickness of the epidermis, with a resulting change in the permeability of SLS. When such an effect differs between subjects it could explain the lack of agreement between the acute and the repeated tests. At present, a brief irritation test will, in all likelihood, be unable to assess an individual's susceptibility to occupational contact dermatitis.
对于检测个体获得职业性接触性皮炎易感性的方法的预测价值，人们了解甚少。最近有人提出为此目的采用诱导刺激后的恢复率。虽然反复接触月桂醇硫酸酯钠（SLS）可能比单次应用更可取，但几乎没有证据支持这一观点。同样，对于简短试验能否预测反复SLS试验的结果也知之甚少。
我们研究了反复SLS试验后的皮肤反应与两项简短试验之间的关系，并特别关注恢复率。
对29名健康志愿者，每周3天，每次6小时，连续3周，分别涂抹0.03%、0.1%和0.3%的SLS，测量经表皮水分流失（TEWL）和红斑情况。将这些数据与涂抹0.1%、0.3%和1.0%的SLS 24小时后的效果以及胶带剥离10次和15次后的效果进行比较。
当使用TEWL增加量（分别为r = 0.04和0.26）或其恢复率（分别为r = - 0.01和0.42）时，反复试验与简短SLS试验或胶带剥离之间的相关性较差。
我们推测，在足够长时间的反复试验中，会出现一些简短试验中不存在的额外机制，例如表皮厚度的改变，从而导致SLS渗透性的变化。当这种效应在个体之间存在差异时，这可能解释急性试验和反复试验之间缺乏一致性的原因。目前，简短刺激试验很可能无法评估个体对职业性接触性皮炎的易感性。
