Jung K, Ha Y, Ha S-K, Han D U, Kim D-W, Moon W K, Chae C
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Building no. 85, San 56-1, Shillim-dong, Kwanak-Gu 151-742.
J Vet Med B Infect Dis Vet Public Health. 2004 Mar;51(2):72-6. doi: 10.1111/j.1439-0450.2004.00732.x.
The aim of these experiments was to investigate the potential antiviral effect of Saccharomyces cerevisiae beta-glucan on the pneumonia induced by swine influenza virus (SIV). Forty colostrum-deprived 5-day-old piglets were randomly divided into four groups of 10. The 20 pigs in groups 1 and 2 were administered Saccharomyces cerevisiae beta-glucan orally (50 mg/day/pig; En-Bio Technology Co., Ltd) for 3 days before SIV infection and those in groups 3 and 4 were given culture medium/diluent alone. Groups 1 and 3 were inoculated intranasally with 3 ml of tissue culture fluid containing 2 x 10(6) tissue culture infective doses 50% (TCID(50))/ml of SIV and those in groups 2 and 4 were exposed in the same manner to uninfected cell culture supernatant. The microscopic lung lesions induced by SIV infection (group 1 pigs) were significantly more severe than those induced by infection in animals pre-administered beta-glucan (group 3) (P < 0.05). Significantly more SIV nucleic acid was detected in the lungs of pigs experimentally infected with SIV only (group 1) at 5, 7 and 10 days post-inoculation (dpi) compared with lungs from pigs pre-administered beta-glucan and infected with SIV (group 3) (P < 0.05). The concentrations of interferon-gamma (IFN-gamma) and nitric oxide (NO) in bronchoalveolar lavage fluid from pigs pre-administered beta-glucan and infected with SIV (group 3) were significantly higher than for any other group at 7 and 10 dpi for IFN-gamma, and at 5, 7 and 10 dpi for NO (P < 0.05). Saccharomyces cerevisiae beta-glucan reduced the pulmonary lesion score and viral replication rate in SIV-infected pigs. These findings support the potential application of beta-glucan as prophylactic/treatment agent in influenza virus infection.
这些实验的目的是研究酿酒酵母β-葡聚糖对猪流感病毒（SIV）诱导的肺炎的潜在抗病毒作用。40头5日龄初乳缺乏仔猪被随机分为4组，每组10头。第1组和第2组的20头猪在感染SIV前3天口服酿酒酵母β-葡聚糖（50毫克/天/头；恩比奥生物技术有限公司），第3组和第4组只给予培养基/稀释剂。第1组和第3组经鼻接种3毫升含有2×10⁶组织培养感染剂量50%（TCID₅₀）/毫升SIV的组织培养液，第2组和第4组以同样方式接触未感染的细胞培养上清液。SIV感染诱导的肺部微观病变（第1组猪）比预先给予β-葡聚糖的动物感染诱导的病变（第3组）严重得多（P<0.05）。与预先给予β-葡聚糖并感染SIV的猪（第3组）的肺相比，在接种后5、7和10天（dpi），仅实验性感染SIV的猪（第1组）的肺中检测到的SIV核酸明显更多（P<0.05）。预先给予β-葡聚糖并感染SIV的猪（第3组）支气管肺泡灌洗液中γ-干扰素（IFN-γ）和一氧化氮（NO）的浓度在7和10 dpi时对于IFN-γ以及在5、7和10 dpi时对于NO均显著高于其他任何组（P<0.05）。酿酒酵母β-葡聚糖降低了SIV感染猪的肺部病变评分和病毒复制率。这些发现支持β-葡聚糖作为流感病毒感染的预防/治疗剂的潜在应用。
