Prieto J M, Giner R M, Recio M C, Máñez S, Ríos J L
Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Avenida Vicent Andrés Estellés, s/n. 46100 Burjassot, Valencia, Spain.
Fitoterapia. 2004 Mar;75(2):179-86. doi: 10.1016/j.fitote.2003.12.004.
The effect of the methanol extract from aerial parts of Bupleurum fruticescens on the release of eicosanoids and hydrolytic enzymes was determined on in vitro cell systems. The extract had a significant effect on 5-lipoxygenase (5-LOX) activity, inhibiting both LTB4 and 5(S)-HETE production with IC50 values of 112 microg/ml and 95 microg/ml, respectively. At concentrations of 200 microg/ml, the extract also inhibited cyclooxygenase-1 (90%) and elastase activities (54%). The 12-LOX activity in intact platelets was not affected; a fact, which suggests that phospholipase A2 (PLA2) activity, is not modified by the extract.
测定了柴胡地上部分甲醇提取物对体外细胞系统中类二十烷酸和水解酶释放的影响。该提取物对5-脂氧合酶（5-LOX）活性有显著影响，分别以112μg/ml和95μg/ml的IC50值抑制LTB4和5(S)-HETE的生成。在200μg/ml的浓度下，该提取物还抑制了环氧合酶-1（90%）和弹性蛋白酶活性（54%）。完整血小板中的12-LOX活性未受影响；这一事实表明，提取物不会改变磷脂酶A2（PLA2）的活性。
