Niethammer Philipp, Bastiaens Philippe, Karsenti Eric
European Molecular Biology Laboratory, EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Science. 2004 Mar 19;303(5665):1862-6. doi: 10.1126/science.1094108.
The spatial organization of the microtubule cytoskeleton is thought to be directed by steady-state activity gradients of diffusible regulatory molecules. We visualized such intracellular gradients by monitoring the interaction between tubulin and a regulator of microtubule dynamics, stathmin, using a fluorescence resonance energy transfer (FRET) biosensor. These gradients were observed both during interphase in motile membrane protrusions and during mitosis around chromosomes, which suggests that a similar mechanism may contribute to the creation of polarized microtubule structures. These interaction patterns are likely to reflect phosphorylation of stathmin in these areas.
微管细胞骨架的空间组织被认为是由可扩散调节分子的稳态活性梯度所引导。我们通过使用荧光共振能量转移（FRET）生物传感器监测微管蛋白与微管动力学调节剂——微管相关蛋白1（stathmin）之间的相互作用，来可视化这种细胞内梯度。在运动性膜突出的间期以及有丝分裂期间围绕染色体的过程中均观察到了这些梯度，这表明类似的机制可能有助于形成极化的微管结构。这些相互作用模式可能反映了这些区域中微管相关蛋白1的磷酸化情况。
