静脉注射甲基黄嘌呤对兔气管上皮中含有岩藻糖基化糖缀合物的杯状细胞比例的影响。

The effects of intravenously administered methylxanthines on the proportion of goblet cells containing fucosylated glycoconjugates in rabbit tracheal epithelium.

作者信息

Vajner Ludek, Konrádová Václava, Uhlík Jirí, Adásková Jana

机构信息

Institute of Histology and Embryology, Second Medical Faculty, Charles University, V Uvalu 84, Prague 5-Motol, CZ-15006, Czech Republic.

出版信息

Acta Histochem. 2004 Feb;106(1):47-54. doi: 10.1016/j.acthis.2003.10.002.

DOI:10.1016/j.acthis.2003.10.002

PMID:15032328

Abstract

The proportion of goblet cells containing various fucosylated glycoconjugates was evaluated with the use of lectin histochemistry in rabbit tracheal epithelium at 15 and 30 min after intravenous administration of either aminophylline (Syntophyllin) or a mixture of etophylline and theophylline (Oxantil). Methylxanthine derivatives are nonspecific inhibitors of phosphodiesterases that are used to treat bronchial asthma; the proportion of fucosylated glycoconjugates strongly affects rheologic properties of respiratory tract mucus. It is concluded that administration of Syntophyllin dramatically lowered the proportion of goblet cells containing fucosylated glycoconjugates in rabbit tracheal epithelium, especially at 30 min after exposure. This decrease was strongest in the levels of alpha(1-2)-fucosylated glycoconjugates. Therefore, Syntophyllin substantially altered the composition and viscoelastic properties of mucus of the upper respiratory tract. The vasodilator Oxantil exerted less pronounced changes in the proportion of goblet cells, but the strongest effect was again found in the levels of alpha(1-2)-fucosylated glycoconjugates.

摘要

在静脉注射氨茶碱（Syntophyllin）或乙羟茶碱与茶碱的混合物（Oxantil）后15分钟和30分钟，利用凝集素组织化学方法评估了兔气管上皮中含有各种岩藻糖基化糖缀合物的杯状细胞比例。甲基黄嘌呤衍生物是用于治疗支气管哮喘的磷酸二酯酶非特异性抑制剂；岩藻糖基化糖缀合物的比例强烈影响呼吸道黏液的流变学特性。得出的结论是，Syntophyllin的给药显著降低了兔气管上皮中含有岩藻糖基化糖缀合物的杯状细胞比例，尤其是在暴露后30分钟。这种降低在α(1-2)-岩藻糖基化糖缀合物水平上最为明显。因此，Syntophyllin显著改变了上呼吸道黏液的组成和黏弹性特性。血管扩张剂Oxantil对杯状细胞比例的影响较小，但在α(1-2)-岩藻糖基化糖缀合物水平上再次发现了最强的作用。