el Walily A F, el-Anwar F, Eid M A, Awaad H
Department of Analytical Pharmaceutical Chemistry, Faculty of Pharmacy, University of Alexandria, Egypt.
Analyst. 1992 Jun;117(6):981-4. doi: 10.1039/an9921700981.
Two rapid assay procedures based on high-performance liquid chromatography (HPLC) and derivative ultraviolet (UV) spectrophotometry have been developed for the simultaneous determination of amoxycillin and dicloxacillin in two-component capsule formulations. The HPLC determination was carried out on a reversed-phase C8 column with use of a mobile phase consisting of methanol-0.02 mol dm-3 ammonium acetate (pH 5) (50 + 50) at a flow rate of 1.0 cm3 min-1, with UV detection at 230 nm. The fourth-derivative spectrophotometric procedure depends on the measurement of the derivative amplitudes, in 0.1 mol dm-3 NaOH, at 308.5 and 275 nm for amoxycillin and dicloxacillin, respectively. For both procedures, the calibration graphs were linear in the ranges 20-200 and 20-140 micrograms cm-3 for the HPLC and UV derivative methods, respectively, with an almost zero intercept and a correlation coefficient of 0.999. Commercial capsules and laboratory-prepared mixtures containing both penicillins in different proportions were assayed by the developed procedures. The results were of comparable accuracy as indicated by a statistical analysis of the data, using both t- and F-tests.
已开发出两种基于高效液相色谱（HPLC）和导数紫外（UV）分光光度法的快速测定方法，用于同时测定两组分胶囊制剂中的阿莫西林和双氯西林。HPLC测定在反相C8柱上进行，使用由甲醇 - 0.02 mol dm⁻³乙酸铵（pH 5）（50 + 50）组成的流动相，流速为1.0 cm³ min⁻¹，在230 nm处进行紫外检测。四阶导数分光光度法取决于在0.1 mol dm⁻³ NaOH中分别在308.5和275 nm处测量阿莫西林和双氯西林的导数幅度。对于这两种方法，HPLC和UV导数法的校准曲线分别在20 - 200和20 - 140微克 cm⁻³范围内呈线性，截距几乎为零，相关系数为0.999。用所开发的方法对含有不同比例两种青霉素的市售胶囊和实验室制备的混合物进行了测定。使用t检验和F检验对数据进行统计分析，结果表明具有相当的准确性。
