Abdel-Moety E M, Abounassif M A, Gad-Kariem el-R A, Khattab N A
Pharmaceutical Chemistry Department, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh-11451, Saudi Arabia.
Talanta. 1993 Jun;40(6):811-7. doi: 10.1016/0039-9140(93)80035-p.
Direct potentiometric titration and two HPLC conditions for the simultaneous determination of amoxycillin and dicloxacillin in their capsules have been developed. One-run titration utilizing 0.05M acet. HClO(4) enables the quantification of both antibiotics. The HPLC-separation could be undertaken on reversed phase, LiChrosorb RP-18 (10 mum), and LiChrospher 100 RP-18 (5 mum), columns by using mobile phases containing acetonitrile + 1% aq, acetic acid, in proportions of 47:53 or 39:61 (v/v), respectively, at a flow rate of 1.5 ml/min with UV-detection at 240 nm. Recoveries of the individual drugs by the application of each described method were found to be fairly satisfactory.
已开发出直接电位滴定法和两种高效液相色谱条件，用于同时测定阿莫西林和双氯西林胶囊中的含量。采用0.05M乙酸高氯酸进行一次滴定即可对两种抗生素进行定量。高效液相色谱分离可在反相LiChrosorb RP - 18（10μm）和LiChrospher 100 RP - 18（5μm）柱上进行，分别使用含乙腈 + 1% 水相乙酸的流动相，比例分别为47:53或39:61（v/v），流速为1.5 ml/min，在240 nm处进行紫外检测。通过应用每种所述方法，发现各药物的回收率相当令人满意。
