Luque E, Peña J, Alonso P J, Jimena I
Department of Morphological Sciences, Histology and Embryology, Faculty of Medicine, University of Córdoba, Spain.
Ann Anat. 1992 Jun;174(3):245-9. doi: 10.1016/s0940-9602(11)80361-4.
Quantitative methods were used to analyze changes in the capillarization of skeletal muscle during regeneration. The H-E staining technique was used to establish three arbitrary stages of fiber regeneration on the basis of qualitative histological evaluation. ATPase and light microscopic techniques were used to calculate capillary density, the number of capillaries surrounding each fiber, and the fiber area served by each capillary, for each of the three stages established. The results obtained indicated variations in the vascularization of regenerating fibers from one stage of maturity of another: capillarization was greater in the initial stages of regeneration, returning gradually to normal values by the final stages of the process. Interestingly enough the pattern in the capillarization of skeletal muscle during regeneration seems to be similar to that taking place during postnatal muscle development. Both processes appear to be governed by the increase in fiber size.
采用定量方法分析骨骼肌再生过程中毛细血管化的变化。基于定性组织学评估，使用苏木精-伊红（H-E）染色技术确定纤维再生的三个任意阶段。对于所确定的三个阶段中的每一个阶段，均使用ATP酶和光学显微镜技术计算毛细血管密度、围绕每条纤维的毛细血管数量以及每条毛细血管所服务的纤维面积。所得结果表明，再生纤维的血管化在不同成熟阶段存在差异：在再生初期毛细血管化程度较高，在该过程的最后阶段逐渐恢复至正常值。有趣的是，骨骼肌再生过程中的毛细血管化模式似乎与出生后肌肉发育过程中的模式相似。这两个过程似乎都受纤维大小增加的支配。
