Jungnickel Julia, Claus Peter, Gransalke Kathleen, Timmer Marco, Grothe Claudia
Department of Neuroanatomy OE 4140, Center of Anatomy, Hannover Medical School, D-30623 Hannover, Germany.
Mol Cell Neurosci. 2004 Mar;25(3):444-52. doi: 10.1016/j.mcn.2003.11.007.
Basic fibroblast growth factor (FGF-2) is involved in the development, maintenance, and survival of the nervous system. To study the physiological role of endogenous FGF-2 during peripheral nerve regeneration, we analyzed sciatic nerves of FGF-2-deleted mice by using morphometric, morphological, and immunocytochemical methods. Quantification of number and size of myelinated axons in intact sciatic nerves revealed no difference between wild-type and FGF-2 knock-out (ko) animals. One week after nerve crush, FGF-2 ko mice showed about five times more regenerated myelinated axons with increased myelin and axon diameter in comparison to wild-types close to the injury site. In addition, quantitative distribution of macrophages and collapsed myelin profiles suggested faster Wallerian degeneration in FGF-2-deleted mice close to the lesion site. Our results suggest that endogenous FGF-2 is crucially involved in the early phase of peripheral nerve regeneration possibly by regulation of Schwann cell differentiation.
碱性成纤维细胞生长因子（FGF - 2）参与神经系统的发育、维持和存活。为研究内源性FGF - 2在周围神经再生过程中的生理作用，我们运用形态测量学、形态学和免疫细胞化学方法分析了FGF - 2基因敲除小鼠的坐骨神经。对完整坐骨神经中有髓轴突的数量和大小进行定量分析，结果显示野生型和FGF - 2基因敲除（ko）动物之间并无差异。神经挤压一周后，与损伤部位附近的野生型小鼠相比，FGF - 2基因敲除小鼠的再生有髓轴突数量多出约五倍，且髓鞘和轴突直径增大。此外，巨噬细胞的定量分布和崩解的髓鞘轮廓表明，在损伤部位附近，FGF - 2基因敲除小鼠的华勒氏变性更快。我们的研究结果表明，内源性FGF - 2可能通过调节雪旺细胞分化，在周围神经再生的早期阶段发挥关键作用。
