Nakagawa Tatsunori, Nakagawa Satoshi, Inagaki Fumio, Takai Ken, Horikoshi Koki
Subground Animalcule Retrieval (SUGAR) Project, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
FEMS Microbiol Lett. 2004 Mar 19;232(2):145-52. doi: 10.1016/S0378-1097(04)00044-8.
The phylogenetic diversity of sulfate-reducing prokaryotes occurring in active deep-sea hydrothermal vent chimney structures was characterized based on the deduced amino acid sequence analysis of the polymerase chain reaction-amplified dissimilatory sulfite reductase (DSR) gene. The DSR genes were successfully amplified from microbial assemblages of the chimney structures, derived from three geographically and geologically distinct deep-sea hydrothermal systems in the Central Indian Ridge (CIR), in the Izu-Bonin Arc (IBA), and the Okinawa Trough (OT), respectively. Phylogenetic analysis revealed seven major phylogenetic groups. More than half of the clones from the CIR chimney structure were related to DSR amino acid sequences of the hyperthermophilic archaeal members of the genus Archaeoglobus, and those of environmental DSR clones within the class Thermodesulfobacteria. From the OT chimney structure, a different group was obtained, which comprised a novel, deep lineage associated with the DSRs of the thermophilic sulfate-reducing bacterium Thermodesulfovibrio. Most of the DSR clones from the IBA chimney structure were phylogenetically associated with the delta-proteobacterial sulfate-reducing bacteria represented by the genus Desulfobulbus. Sequence analysis of DSR clones demonstrated a diverse sulfate-reducing prokaryotic community in the active deep-sea hydrothermal chimney structures.
基于聚合酶链反应扩增的异化亚硫酸盐还原酶（DSR）基因推导的氨基酸序列分析，对活跃深海热液喷口烟囱结构中存在的硫酸盐还原原核生物的系统发育多样性进行了表征。分别从位于中印度洋海岭（CIR）、伊豆-小笠原弧（IBA）和冲绳海槽（OT）的三个地理和地质上不同的深海热液系统的烟囱结构微生物群落中成功扩增出DSR基因。系统发育分析揭示了七个主要的系统发育组。来自CIR烟囱结构的克隆中，超过一半与嗜热古菌属嗜热栖热菌的DSR氨基酸序列以及热脱硫杆菌纲内的环境DSR克隆相关。从OT烟囱结构中获得了一个不同的组，它包含一个与嗜热硫酸盐还原菌嗜热脱硫弧菌的DSR相关的新的深层谱系。来自IBA烟囱结构的大多数DSR克隆在系统发育上与以脱硫球茎菌属为代表的δ-变形菌纲硫酸盐还原菌相关。DSR克隆的序列分析表明，活跃深海热液烟囱结构中存在多样化的硫酸盐还原原核生物群落。
