Kormas Konstantinos A, Tivey Margaret K, Von Damm Karen, Teske Andreas
Department of Animal Production and Aquatic Environment, University of Thessaly, 383 34 N, Ionia, Volos, Greece.
Environ Microbiol. 2006 May;8(5):909-20. doi: 10.1111/j.1462-2920.2005.00978.x.
A diffusely venting chimney spire from the East Pacific Rise (9 degrees N) was analysed by petrographic thin sectioning and 16S rRNA gene cloning and sequencing in parallel, to correlate microbial community composition with mineralogy and inferred in situ conditions within the chimney mineral matrix. Both approaches indicated a zonation of the chimney spire into distinct microhabitats for different bacteria and archaea. The thermal gradient inferred from the mineral composition and porosity of the chimney was consistent with the distribution of bacterial and archaeal phylotypes in the chimney matrix. A novel phylogenetic lineage of euryarchaeota was found that co-occurred with clones related to cultured hyperthermophilic archaea. A few phylotypes related to mesophilic bacteria were found in the hot core of the chimney, indicating that seawater influx during retrieval and cooling of these highly porous structures can entrain microorganisms into chimney layers that are not their native habitat.
通过岩石学薄片切片以及16S rRNA基因克隆和测序并行分析了东太平洋海隆（北纬9度）一个弥漫排气的烟囱尖顶，以关联微生物群落组成与烟囱矿物基质内的矿物学及推断的原位条件。两种方法均表明烟囱尖顶被划分为不同细菌和古菌的独特微生境。从烟囱的矿物成分和孔隙率推断出的热梯度与烟囱基质中细菌和古菌系统发育型的分布一致。发现了一个新的广古菌门系统发育谱系，它与培养的嗜热古菌相关克隆同时出现。在烟囱的热核心中发现了一些与嗜温细菌相关的系统发育型，这表明在这些高度多孔结构的取回和冷却过程中海水涌入会将微生物带入并非其原生栖息地的烟囱层。
