采用柱后衍生高效液相色谱法分析挪威贻贝中的麻痹性贝类毒素。

Analysis of PSP toxins in Norwegian mussels by a post-column derivatization HPLC method.

作者信息

Asp T N, Larsen S, Aune T

机构信息

Section of Food Hygiene, Norwegian School of Veterinary Science, P.O. Box 8146 Dep., 0033 Oslo, Norway.

出版信息

Toxicon. 2004 Mar 1;43(3):319-27. doi: 10.1016/j.toxicon.2004.01.004.

DOI:10.1016/j.toxicon.2004.01.004

PMID:15033331

Abstract

The aim of this study was to investigate the agreement between the AOAC mouse bioassay and an HPLC method for determining Paralytic Shellfish Poisoning (PSP) toxicity in blue mussels from the Norwegian coast. The AOAC mouse bioassay has traditionally been used for determining the toxin levels. Recently, an HPLC method for determining PSP toxins has been implemented and run in parallel with the bioassay. Four hundred mussel extracts from the last six years were analysed with both methods. A highly significant linear correlation between the methods was achieved (r = 0.84). However, the relation between the two methods was best described by a second-degree polynom. Totally, this model explained 87% of the variation in the HPLC method. By agreement analysis, it was possible to establish cut-off levels for the HPLC method related to the AOAC mouse bioassay with kappa values >0.80 for toxicity levels below 500 MU/100 g. The HPLC method could substitute the mouse bioassay in determining PSP toxicity in Norwegian mussels. Additionally, about 70 mussel samples were also analysed by the MIST Alert trade mark test kit. The kit did not give any false negative results compared with the regulatory limit, but 30% of the samples below the cut point were also determined as positive.

摘要

本研究的目的是调查美国官方分析化学师协会（AOAC）小鼠生物测定法与一种高效液相色谱（HPLC）方法之间的一致性，该HPLC方法用于测定挪威沿海蓝贻贝中的麻痹性贝类毒素（PSP）毒性。传统上，AOAC小鼠生物测定法一直用于确定毒素水平。最近，一种用于测定PSP毒素的HPLC方法已实施并与生物测定法并行运行。用这两种方法分析了过去六年中采集的400份贻贝提取物。两种方法之间获得了高度显著的线性相关性（r = 0.84）。然而，两种方法之间的关系最好用二次多项式来描述。总体而言，该模型解释了HPLC方法中87%的变异。通过一致性分析，有可能为HPLC方法确定与AOAC小鼠生物测定法相关的临界值，对于毒性水平低于500 MU/100 g的情况，kappa值>0.80。HPLC方法可以替代小鼠生物测定法来测定挪威贻贝中的PSP毒性。此外，大约70份贻贝样品还通过MIST Alert商标检测试剂盒进行了分析。与监管限值相比，该试剂盒未给出任何假阴性结果，但低于临界点的样品中有30%也被判定为阳性。