Boutet Isabelle, Tanguy Arnaud, Moraga Dario
Laboratoire des Sciences de l'Environnement Marin (LEMAR), UMR-CNRS 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Tech. Brest Iroise, Place Nicolas Copernic, 29280 Plouzané, France.
Gene. 2004 Mar 31;329:147-57. doi: 10.1016/j.gene.2003.12.027.
Hydrocarbon contamination perturbs the metabolism of the marine bivalve Crassostrea gigas. To understand the response of this organism to hydrocarbon exposure, a suppression subtractive hybridisation method was employed to characterise up- and down-regulated genes during hydrocarbon exposure. The number of differentially expressed gene sequences obtained via this method was 258. The expression of genes involved in hydrocarbon detoxification (cytochrome p4501A1-like protein, cytochrome b(5), flavin-containing monooxygenase 2 and glutathione S-transferase omega class), protection against oxidative stress (copper/zinc superoxide dismutase) and cell protection (heat shock protein 70 family) was analysed by RT-PCR. An increase in the mRNA level of all genes studied was observed. A quantification of HSP70 by Enzyme Linked Immunosorbent Assay (ELISA) showed a significant increase of this protein during exposure. This study provides a basis for studying hydrocarbon detoxification processes in marine bivalves, especially C. gigas.
碳氢化合物污染会扰乱海洋双壳贝类太平洋牡蛎的新陈代谢。为了解这种生物对碳氢化合物暴露的反应，采用抑制性消减杂交方法来鉴定碳氢化合物暴露期间上调和下调的基因。通过该方法获得的差异表达基因序列数量为258个。采用逆转录聚合酶链反应（RT-PCR）分析了参与碳氢化合物解毒（细胞色素p4501A1样蛋白、细胞色素b(5)、含黄素单加氧酶2和谷胱甘肽S-转移酶ω类）、抗氧化应激保护（铜/锌超氧化物歧化酶）和细胞保护（热休克蛋白70家族）的基因表达。观察到所有研究基因的mRNA水平均有所增加。通过酶联免疫吸附测定（ELISA）对热休克蛋白70（HSP70）进行定量分析，结果显示暴露期间该蛋白显著增加。本研究为研究海洋双壳贝类尤其是太平洋牡蛎的碳氢化合物解毒过程提供了依据。
