Moraga Dario, Meistertzheim Anne-Leïla, Tanguy-Royer Séverine, Boutet Isabelle, Tanguy Arnaud, Donval Anne
Laboratoire des Sciences de l'Environnement Marin (LEMAR), UMR-CNRS 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Place Nicolas Copernic, F-29280, Plouzané, France.
Comp Biochem Physiol C Toxicol Pharmacol. 2005 Jun;141(2):151-6. doi: 10.1016/j.cca.2005.05.014.
Localization of heat shock proteins (HSPs) and metallothioneins (MTs) was investigated in a marine bivalve (Crassostrea gigas) by immunohistochemical methods. Differential protein expression was demonstrated in digestive gland, gonad and gills, using a polyclonal antibody against C. gigas proteins. Application of this technique showed the cellular and tissue immunolabelling specificity of the two proteins. HSPs and MTs were localized in the epithelium of the digestive gland and gills in contact with the palleal compartment. For the first time, localization of MTs was observed in mature gametes of bivalve molluscs. Our results establish a basis for the use of immmunodetection techniques to study the tissue-specific localization of stress proteins in marine bivalves exposed to metal stress.
采用免疫组织化学方法研究了海洋双壳贝类（太平洋牡蛎）中热休克蛋白（HSPs）和金属硫蛋白（MTs）的定位。使用针对太平洋牡蛎蛋白的多克隆抗体，在消化腺、性腺和鳃中证明了蛋白质表达的差异。该技术的应用显示了这两种蛋白质的细胞和组织免疫标记特异性。HSPs和MTs定位于与外套腔接触的消化腺和鳃的上皮细胞中。首次在双壳贝类的成熟配子中观察到MTs的定位。我们的结果为利用免疫检测技术研究暴露于金属胁迫下的海洋双壳贝类应激蛋白的组织特异性定位奠定了基础。
