Walsh William R, Loefler Andreas, Nicklin Sean, Arm Doug, Stanford Ralph E, Yu Yan, Harris Richard, Gillies R M
Orthopaedic Research Laboratories, Prince of Wales Hospital, University of New South Wales, Sydney, Australia.
Eur Spine J. 2004 Jul;13(4):359-66. doi: 10.1007/s00586-003-0597-9. Epub 2004 Mar 18.
Augmenting healing through a single application of an exogenous growth factor or bone morphogenetic protein is not a new concept. The use of autologous growth factors through platelet isolation and concentration provides multiple endogenous growth factors to the healing site. A posterolateral fusion model in aged sheep (5- to 6-year-old ewes) was used to examine the effects of the addition of growth factors through autologous platelet isolation on the biomechanic and histologic properties of the fusion using a resorbable coral bone graft substitute. At 6 months the combination of autologous growth factors to the Pro Osteon 500R plus aspirated bone marrow resulted in the greatest bending stiffness but not ultimate load. Autologous growth factors can be isolated from platelets and concentrated to provide multiple growth factors to the fusion site to aid in spinal fusion.
通过单次应用外源性生长因子或骨形态发生蛋白来促进愈合并非新概念。通过血小板分离和浓缩使用自体生长因子可向愈合部位提供多种内源性生长因子。采用老年绵羊（5至6岁母羊）的后外侧融合模型，使用可吸收珊瑚骨移植替代物，研究通过自体血小板分离添加生长因子对融合的生物力学和组织学特性的影响。6个月时，将自体生长因子与Pro Osteon 500R加抽取的骨髓相结合，产生了最大的弯曲刚度，但未产生最大极限载荷。自体生长因子可从血小板中分离并浓缩，以向融合部位提供多种生长因子，有助于脊柱融合。
