Rodrigues Katia F, Sieber Thomas N, Grünig Christoph R, Holdenrieder Ottmar
FIOCRUZ, Instituto Oswaldo Cruz, Departamento de Micologia, CP 926, BR-21045-900 Rio de Janeiro, RJ, Brazil.
Mycol Res. 2004 Jan;108(Pt 1):45-52. doi: 10.1017/s0953756203008840.
Phenotypic and genotypic variability of 18 endophytic Guignardia strains from different host plants (Anacardiaceae: Anacardium giganteum, Myracrodruon urundeuva, Spondias mombin; Apocynaceae: Aspidosperma polyneuron; Ericaceae: Rhododendron sp.; Fabaceae: Bowdichia nitida; Leguminosae: Cassia occidentalis; Rutaceae: Citrus aurantium) growing in different sites in Brazil was assessed by means of morphometric measurements and inter-single-sequence-repeat-anchored polymerase chain reaction (ISSR-PCR) amplifications of the DNA. Morphology of conidia and ascospores and growth rates of the Brazilian isolates corresponded well with those of G. mangiferae. Multiple correspondence analysis (MCA) of the ISSR-PCR data yielded three groups of strains, which did, however, not correspond either to the host or to the geographic origin. The same individual tree hosted genotypically different strains indicating multiple infections. Phylogeny based on ITS1-5.8S-ITS2 sequence analyses confirmed conspecificity of the Brazilian isolates with G. mangiferae (syn. G. endophyllicola).
通过形态测量以及对DNA进行单序列重复锚定聚合酶链反应（ISSR-PCR）扩增，评估了来自巴西不同地点、生长于不同寄主植物（漆树科：巨籽棕、乌伦杜瓦巴西苏木、南酸枣；夹竹桃科：多脉白坚木；杜鹃花科：杜鹃属；豆科：亮叶鲍迪豆；豆科：西番莲；芸香科：酸橙）的18株内生拟盘多毛孢菌株的表型和基因型变异性。巴西分离株的分生孢子和子囊孢子形态以及生长速率与芒果拟盘多毛孢的形态和生长速率非常吻合。对ISSR-PCR数据进行的多重对应分析（MCA）产生了三组菌株，但这些菌株既不与寄主对应，也不与地理来源对应。同一棵树上存在基因型不同的菌株，表明存在多重感染。基于ITS1-5.8S-ITS2序列分析的系统发育证实了巴西分离株与芒果拟盘多毛孢（同义词：内生拟盘多毛孢）的同种性。
