Asrat D, Nilsson I, Mengistu Y, Ashenafi S, Ayenew K, Al-Soud W Abu, Wadström T, Kassa E
Department of Medical Microbiology, Immunology and Parasitology, Faculty of Medicine, Addis Ababa University, P.O. Box 9086, Addis Ababa, Ethiopia.
Ann Trop Med Parasitol. 2004 Mar;98(2):181-9. doi: 10.1179/000349804225003190.
In developing countries such as Ethiopia, where chronic gastritis and peptic-ulcer disease are the most common endoscopic findings, it is important to study the association between Helicobacter pylori infection and gastroduodenal diseases. Both invasive and non-invasive diagnostic methods were therefore used to investigate 300, consecutive, adult patients with dyspepsia, from the gastrointestinal clinic of Tikur Anbassa University Hospital, Addis Ababa. The apparent overall prevalence of H. pylori infection varied according to the detection method employed. Culture revealed H. pylori in only 69% of the patients but this pathogen appeared more common when rapid urease tests (71%), PCR-denaturating gradient gel electrophoresis (91%), histopathology (81%), silver staining (75%) or stool-antigen tests (81%) were employed. Antibodies to H. pylori were detected, both by enzyme immuno-assay (EIA) and immunoblotting, in approximately 80% of the patients, whether the antigens used were of a reference strain or from a local isolate of H. pylori. When some of the EIA-positive and EIA-negative sera were cross-absorbed with antigens of Campylobacter jejuni and re-tested by EIA, the H. pylori-positive sera remained positive and the negative sera remained negative. Dyspeptic patients in Ethiopia, like most of those previously observed elsewhere in Africa, are often infected with H. pylori. It is important that the management of these patients should not be hampered by the misinterpretation of the African epidemiology of this pathogen.
在诸如埃塞俄比亚这样的发展中国家，慢性胃炎和消化性溃疡疾病是最常见的内镜检查结果，研究幽门螺杆菌感染与胃十二指肠疾病之间的关联很重要。因此，采用侵入性和非侵入性诊断方法对来自亚的斯亚贝巴提库尔·安巴萨大学医院胃肠科的300例连续成年消化不良患者进行了调查。幽门螺杆菌感染的表观总体患病率因所采用的检测方法而异。培养仅在69%的患者中发现幽门螺杆菌，但当采用快速尿素酶试验（71%）、聚合酶链反应-变性梯度凝胶电泳（91%）、组织病理学（81%）、银染色（75%）或粪便抗原检测（81%）时，这种病原体似乎更为常见。无论所用抗原是参考菌株还是幽门螺杆菌的本地分离株，通过酶免疫测定（EIA）和免疫印迹法在大约80%的患者中检测到了幽门螺杆菌抗体。当一些EIA阳性和EIA阴性血清用空肠弯曲菌抗原交叉吸收并通过EIA重新检测时，幽门螺杆菌阳性血清仍为阳性，阴性血清仍为阴性。埃塞俄比亚的消化不良患者与之前在非洲其他地方观察到的大多数患者一样，经常感染幽门螺杆菌。重要的是，这些患者的治疗不应因对这种病原体在非洲流行情况的错误解读而受到阻碍。
