Yaekashiwa Noriko, Tamate Hidetoshi B, Takeuchi Tomoko, Sugimoto Haruyo, Shibata Kiyotaka, Kinemuchi Hiroyasu
School of Science and Engineering, Senshu University at Ishinomaki, Ishinomaki 986-8580, Japan.
Inflammopharmacology. 2003;11(2):145-54. doi: 10.1163/156856003765764317.
To study the molecular structure of guinea pig monoamine oxidase (MAO) and its phylogenetic relationship with other mammalian MAOs, we determined nucleotide sequences of putative MAO cDNAs isolated from guinea pig tissues. Both the 5'- and 3'-ends of the cDNAs were amplified using the RACE (rapid amplification of cDNA ends) method. The sequence (1924 bp) of a putative guinea-pig MAO-B cDNA covers a complete coding region that corresponds to 521 amino acids. We also analyzed a partial sequence of a putative guinea-pig MAO-A cDNA, which corresponds to 506 amino acids, but have left the region of 66 bp at the 3'-end undetermined. The nucleotide and deduced amino-acid sequences of the putative guinea-pig MAO cDNAs showed the highest homology with that of human MAO cDNAs among the known mammalian MAO sequences. These results suggest that guinea-pig MAOs are structurally similar to human MAOs. Our molecular phylogenetic data support the idea that guinea pigs and rodents diverged before the separation between rodents and other lineage leading to Primates and Artiodactyla.
为了研究豚鼠单胺氧化酶（MAO）的分子结构及其与其他哺乳动物MAO的系统发育关系，我们测定了从豚鼠组织中分离出的假定MAO cDNA的核苷酸序列。使用RACE（cDNA末端快速扩增）方法扩增了cDNA的5'端和3'端。假定的豚鼠MAO - B cDNA序列（1924 bp）覆盖了一个完整的编码区，对应于521个氨基酸。我们还分析了假定的豚鼠MAO - A cDNA的部分序列，其对应于506个氨基酸，但3'端有66 bp的区域未确定。在已知的哺乳动物MAO序列中，假定的豚鼠MAO cDNA的核苷酸和推导的氨基酸序列与人类MAO cDNA的同源性最高。这些结果表明，豚鼠MAO在结构上与人类MAO相似。我们的分子系统发育数据支持这样一种观点，即豚鼠和啮齿动物在啮齿动物与导致灵长类和偶蹄类的其他谱系分离之前就已经分化。
