Lercker G, Bocci F, Frega N, Bortolomeazzi R
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione di Industrie e Tecnologie Alimentari, Firenze.
Farmaco. 1992 Mar;47(3):367-78.
The cannabinoid pattern of vegetable preparations from Cannabis sativa (hashish, marijuana) allows to recognize the phenotype of the plants, to be used as drug or for fiber. Cannabinoid determination by analytical point of view has represented some problems caused by the complex composition of the hexane extract. Capillary gas chromatography of the hexane extracts of vegetable samples, shows the presence of rather polar constituents that eluted, with noticeable interactions, only on polar phase. The compounds can be methylated by diazomethane and silanized (TMS) by silylating reagents. The methyl and methyl-TMS derivatives are analyzed by high resolution gas chromatography (HRGC) and by gas chromatography-mass spectrometry (GC-MS). The identification of the compounds shows their nature of cannabinoid acids, which the main by quantitative point of view results the cannabidiolic acid (CBDA). It is known that the cannabinoid acids are thermally unstable and are transformed in the corresponding cannabinoids by decarboxilation. This is of interest in forensic analysis with the aim to establish the total amount of THC in the Cannabis preparations, as the active component.
来自大麻（哈希什、大麻）的植物制剂中的大麻素模式有助于识别植物的表型，这些植物可用于制作毒品或获取纤维。从分析角度来看，大麻素的测定存在一些问题，这是由己烷提取物的复杂成分导致的。蔬菜样品己烷提取物的毛细管气相色谱显示，存在相当极性的成分，这些成分仅在极性相上洗脱，且有明显的相互作用。这些化合物可以用重氮甲烷进行甲基化，并通过硅烷化试剂进行硅烷化（TMS）。甲基和甲基-TMS衍生物通过高分辨率气相色谱（HRGC）和气相色谱-质谱联用（GC-MS）进行分析。化合物的鉴定表明它们是大麻素酸的性质，从定量角度来看，主要的是大麻二酚酸（CBDA）。众所周知，大麻素酸热不稳定，会通过脱羧作用转化为相应的大麻素。这在法医分析中很重要，目的是确定大麻制剂中作为活性成分的四氢大麻酚的总量。
