Analytical Chemistry Department, University of the Basque Country (UPV/EHU), Barrio Sarriena s/n, 48940, Leioa, Basque Country, Spain,
Anal Bioanal Chem. 2014 Nov;406(29):7549-60. doi: 10.1007/s00216-014-8177-x. Epub 2014 Oct 23.
High performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) has been successfully applied to cannabis plant extracts in order to identify cannabinoid compounds after their quantitative isolation by means of supercritical fluid extraction (SFE). MS conditions were optimized by means of a central composite design (CCD) approach, and the analysis method was fully validated. Six major cannabinoids [tetrahydrocannabinolic acid (THCA), tetrahydrocannabinol (THC), cannabidiol (CBD), tetrahydrocannabivarin (THCV), cannabigerol (CBG), and cannabinol (CBN)] were quantified (RSD < 10%), and seven more cannabinoids were identified and verified by means of a liquid chromatograph coupled to a quadrupole-time-of-flight (Q-ToF) detector. Finally, based on the distribution of the analyzed cannabinoids in 30 Cannabis sativa L. plant varieties and the principal component analysis (PCA) of the resulting data, a clear difference was observed between outdoor and indoor grown plants, which was attributed to a higher concentration of THC, CBN, and CBD in outdoor grown plants.
高效液相色谱串联质谱法（HPLC-MS/MS）已成功应用于大麻植物提取物，以在通过超临界流体萃取（SFE）定量分离后鉴定大麻素化合物。通过中心复合设计（CCD）方法优化了 MS 条件，并对分析方法进行了全面验证。定量了六种主要大麻素[四氢大麻酸（THCA）、四氢大麻醇（THC）、大麻二酚（CBD）、四氢大麻素（THCV）、大麻萜酚（CBG）和大麻酚（CBN）]（RSD<10%），并通过液相色谱仪与四极杆飞行时间（Q-ToF）检测器联用鉴定和验证了另外七种大麻素。最后，基于分析的大麻素在 30 种大麻属植物品种中的分布以及所得数据的主成分分析（PCA），观察到室外和室内种植的植物之间存在明显差异，这归因于室外种植的植物中 THC、CBN 和 CBD 的浓度更高。
