Pérez-Costas Emma, Meléndez-Ferro Miguel, Pérez-García Carlos G, Caruncho Héctor J, Rodicio María Celina
Departamento de Biología Celular y Ecología, Area de Biología Celular, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain.
J Chem Neuroanat. 2004 Mar;27(1):7-21. doi: 10.1016/j.jchemneu.2003.08.005.
The expression of reelin, a large extracellular matrix glycoprotein, was studied in the brain of pre-spawning adult sea lampreys by immunohistochemistry using two monoclonal antibodies against this protein. Reelin immunoreactive (reln-ir) neurons were observed in the olfactory bulb, and pallial and subpallial regions in the telencephalon. In the diencephalon, reln-ir cells were observed in some hypothalamic nuclei, in the nucleus of Bellonci, and in the habenula. In the mesencephalon, this protein was detected in several nuclei related with the centrifugal visual system, although the optic tectum was devoid of immunoreactivity. The hindbrain showed several nuclei with immunopositive neurons, including the branchiomeric nerve motor nuclei and also some groups of non-giant cells of the reticular formation. The rostral spinal cord showed some immunopositive neurons mainly located in lateral and ventral positions. Overall, the pattern of distribution of reelin in the adult sea lamprey correlates with the previously reported in other adult vertebrates. Furthermore, the wide distribution of reelin in the adult lamprey brain is consistent with a possible existence of different roles for this protein not related with development in the central nervous system (CNS) of vertebrates (i.e. neuronal plasticity and/or maintenance).
利用两种针对细胞外基质大型糖蛋白reelin的单克隆抗体，通过免疫组织化学方法，对产卵前成年海七鳃鳗大脑中该蛋白的表达进行了研究。在嗅球以及端脑的脑皮质和皮质下区域观察到了reelin免疫反应性（reln-ir）神经元。在间脑，在下丘脑的一些核团、贝隆奇核以及缰核中观察到了reln-ir细胞。在中脑，尽管视顶盖没有免疫反应性，但在与离心视觉系统相关的几个核团中检测到了这种蛋白。后脑显示出几个具有免疫阳性神经元的核团，包括鳃弓神经运动核以及网状结构的一些非巨细胞群。脊髓前端显示出一些主要位于外侧和腹侧位置的免疫阳性神经元。总体而言，成年海七鳃鳗中reelin的分布模式与先前在其他成年脊椎动物中报道的情况相关。此外，成年七鳃鳗大脑中reelin的广泛分布与该蛋白在脊椎动物中枢神经系统（CNS）发育无关的不同作用（即神经元可塑性和/或维持）的可能存在相一致。
