Pérez-Costas Emma, Meléndez-Ferro Miguel, Santos Ysabel, Anadón Ramón, Rodicio M Celina, Caruncho Héctor J
Department of Fundamental Biology, Faculty of Biology, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
J Chem Neuroanat. 2002 Mar;23(3):211-21. doi: 10.1016/s0891-0618(01)00156-9.
In order to analyze the presence of a reelin-like protein in the brain of a primitive vertebrate with a laminar-type brain, such as the sea lamprey, Western blot and immunohistochemical approaches were employed by using the G10 and 142 reelin-specific monoclonal antibodies. Western blots of lamprey brain extracts showed bands of about 400 kDa, 180 kDa and others below 100 kDa; similar bands were observed in samples from rat cerebellum. In different larval stages there was a prominent reelin immunolabeling associated with the olfactory bulb, pallial regions, habenula, hypothalamus and optic tectum. In addition, the olfactory and optic tracts, as well as the afferent and efferent (fasciculus retroflexus) tracts of the habenular ganglion, also showed immunopositivity in these stages. Interestingly, the highest level of labeling was observed in premetamorphic larvae, just prior to entering the metamorphic stage. These data indicate that reelin expression is also prominent in brains of primitive vertebrates without layered cortical regions, suggesting that some physiological roles of reelin not related to the regulation of neuronal migration in layered cortical regions (i.e. involvement in axon pathfinding, synaptogenesis, dendritic arborization and neuronal plasticity) might have appeared earlier in evolution.
为了分析在具有层状脑的原始脊椎动物如海七鳃鳗的脑中是否存在类reelin蛋白，使用G10和142种reelin特异性单克隆抗体采用了蛋白质免疫印迹法和免疫组织化学方法。海七鳃鳗脑提取物的蛋白质免疫印迹显示出约400 kDa、180 kDa以及其他低于100 kDa的条带；在大鼠小脑的样本中也观察到了类似的条带。在不同的幼体阶段，嗅球、脑皮质区域、缰核、下丘脑和视顶盖均有显著的reelin免疫标记。此外，嗅神经和视神经束以及缰神经节的传入和传出（后屈束）束在这些阶段也显示出免疫阳性。有趣的是，在即将进入变态阶段的前变态幼体中观察到了最高水平的标记。这些数据表明，reelin表达在没有分层皮质区域的原始脊椎动物的脑中也很突出，这表明reelin的一些与分层皮质区域中神经元迁移调节无关的生理作用（即参与轴突寻路、突触发生、树突分支和神经元可塑性）可能在进化中出现得更早。
