Rokach Joshua, Kim Seongjin, Bellone Sophie, Lawson John A, Praticò Domenico, Powell William S, FitzGerald Garret A
Department of Chemistry, Claude Pepper Institute, Florida Institute of Technology, 150 West University Blvd., Melbourne, FL 32901, USA.
Chem Phys Lipids. 2004 Mar;128(1-2):35-56. doi: 10.1016/j.chemphyslip.2003.09.011.
Isoprostanes (iP's), a new class of natural products isomeric with prostaglandins, are formed as the result of free radical oxygenation of polyunsaturated fatty acids. We have identified these iP's and developed analytical methodology to measure them in biological fluids. The approach we took, which led to the discovery and measurement of iP's, is as follows: (1) based on some biochemical and chemical considerations, we proposed possible structures for these isoprostanes; (2) we performed the total syntheses of some of these iP's, in particular Groups III through VI, and used them as markers for their discovery in biological fluids and developed a GC/MS and an LC/MS methodologies based on iPF2alpha-III, iPF2alpha-VI, and 8,12-iso-iPF2alpha-VI; (3) with the help of these assays, we measured elevated levels of iP's in Alzheimer's disease and atherosclerosis.
异前列腺素（iP's）是一类与前列腺素异构的新型天然产物，由多不饱和脂肪酸的自由基氧化作用形成。我们已经鉴定出这些异前列腺素，并开发了在生物体液中测量它们的分析方法。我们用于发现和测量异前列腺素的方法如下：（1）基于一些生化和化学方面的考虑，我们提出了这些异前列腺素的可能结构；（2）我们完成了其中一些异前列腺素的全合成，特别是第三至第六组，并将它们用作在生物体液中发现它们的标志物，并基于iPF2α-III、iPF2α-VI和8,12-异-iPF2α-VI开发了气相色谱/质谱和液相色谱/质谱方法；（3）借助这些检测方法，我们测量了阿尔茨海默病和动脉粥样硬化患者体内升高的异前列腺素水平。
