Kurul Semra, Kavukcu Salih, Sis Banu, Yilmaz Osman, Sarioglu Sulen, Soylu Alper, Turkmen Mehmet, Dirik Eray
Department of Pediatric Neurology, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
Pediatr Nephrol. 2004 Jun;19(6):621-6. doi: 10.1007/s00467-004-1423-2. Epub 2004 Mar 20.
The aim of this study was to investigate the effect of increased number and frequency of vaccination on immune complex deposition in the choroid plexus and glomeruli of non-nephrectomized and unilateral nephrectomized mice. Fifty-five non-nephrectomized, 40 nephrectomized, and 7 control Swiss albino mice were used. Half of each group was vaccinated only with diphtheria-tetanus and the other half with multiple vaccines, which are used in pediatric practice. Each group was divided into subgroups, which were vaccinated with increasing frequency. No immune deposits were detected in the choroid plexus of any vaccinated mice. There were immune deposits in glomeruli in 2 of 55 (3.6%) of the non-nephrectomized and in 3 of 40 (7.5%) of the nephrectomized mice (P=0.199). The difference between the diphtheria-tetanus and multiple vaccine groups in non-nephrectomized (P=0.236) and nephrectomized (P=1.000) mice was not significant. A significant positive correlation between increased frequency of vaccination and glomerular immune complex deposition 8 weeks after the last immunization was detected in the multiple vaccine group of nephrectomized mice (P=0.048, r=0.447). Our results suggest that the large number and frequent doses of vaccines would not lead to immune complex deposition in choroid plexus and do not significantly increase the deposition of immune complexes in glomeruli in unilateral nephrectomized mice compared with non-nephrectomized mice.
本研究的目的是调查增加疫苗接种次数和频率对未行肾切除术及单侧肾切除小鼠脉络丛和肾小球中免疫复合物沉积的影响。使用了55只未行肾切除术的小鼠、40只肾切除小鼠和7只对照瑞士白化小鼠。每组一半小鼠仅接种白喉-破伤风疫苗，另一半接种儿科常用的多种疫苗。每组再分为若干亚组，以递增频率进行疫苗接种。在任何接种疫苗的小鼠脉络丛中均未检测到免疫沉积物。未行肾切除术的小鼠中，55只中有2只（3.6%）肾小球存在免疫沉积物；肾切除小鼠中，40只中有3只（7.5%）肾小球存在免疫沉积物（P=0.199）。未行肾切除术的小鼠（P=0.236）和肾切除小鼠（P=1.000）中，白喉-破伤风疫苗组和多种疫苗组之间的差异不显著。在肾切除小鼠的多种疫苗组中，检测到最后一次免疫后8周疫苗接种频率增加与肾小球免疫复合物沉积之间存在显著正相关（P=0.048，r=0.447）。我们的结果表明，与未行肾切除术的小鼠相比，大量且频繁接种疫苗不会导致脉络丛中免疫复合物沉积，也不会显著增加单侧肾切除小鼠肾小球中免疫复合物的沉积。
