Adams Ian R, McLaren Anne
Wellcome Trust/Cancer Research UK Institute of Cancer and Developmental Biology, Cambridge, United Kingdom.
Dev Dyn. 2004 Apr;229(4):733-44. doi: 10.1002/dvdy.10471.
We have identified a mouse ortholog of the yeast Rif1 family of telomere-associated proteins on the basis of its high expression in primordial germ cells and embryo-derived pluripotent stem cell lines. mRif1 is also highly expressed in totipotent and pluripotent cells during early mouse development, and in male and female germ cells in adult mice. mRif1 expression is induced during derivation of embryonic stem cells and is rapidly down-regulated upon differentiation of embryonic stem cells in vitro. Furthermore, we show that mRif1 physically interacts with the telomere-associated protein mTrf2 and can be cross-linked to telomeric repeat DNA in mouse embryonic stem cells. mRif1 may be involved in the maintenance of telomere length or pluripotency in the germline and during early mouse development.
基于其在原始生殖细胞和胚胎来源的多能干细胞系中的高表达，我们鉴定出了酵母端粒相关蛋白Rif1家族的小鼠直系同源物。mRif1在小鼠早期发育过程中的全能和多能细胞中以及成年小鼠的雄性和雌性生殖细胞中也高度表达。mRif1的表达在胚胎干细胞的诱导过程中被诱导，并且在体外胚胎干细胞分化时迅速下调。此外，我们表明mRif1与端粒相关蛋白mTrf2发生物理相互作用，并且可以在小鼠胚胎干细胞中与端粒重复DNA交联。mRif1可能参与生殖系和小鼠早期发育过程中端粒长度或多能性的维持。
