评估活体染色剂对离体大鼠视网膜的毒性。
Evaluation of toxicity due to vital stains in isolated rat retinas.
作者信息
Tokuda Kazuhiro, Tsukamoto Teruhisa, Fujisawa Shigeki, Matsubara Masao
机构信息
Department of Ophthalmology, Daini Hospital, Tokyo Women's Medical University, Arakawa, Tokyo, Japan.
出版信息
Acta Ophthalmol Scand. 2004 Apr;82(2):189-94. doi: 10.1111/j.1600-0420.2004.00224.x.
PURPOSE
We investigated whether artificial aqueous humours are adequate incubation media compared with artificial cerebrospinal fluid (aCSF), and evaluated the retinal toxicity of two vital stains--trypan blue (TB) and indocyanine green (ICG)--and triamcinolone acetonide (TA) using isolated rat retinas incubated in artificial aqueous humours.
METHODS
In experiment 1, retinal segments were isolated and incubated in aCSF, BSS plus, Opeguard Neo Kit, or phosphate-buffered saline (PBS). In experiment 2, retinal tissues were exposed to one of the agents and incubated in BSS plus. Retinal damage was assessed by morphological examination and biochemical assay, which measured lactate dehydrogenase (LDH) in the medium once every hour.
RESULTS
In experiment 1, BSS plus was confirmed as a suitable incubation medium. In experiment 2, there were no significant changes in the retinas exposed to TB or TA. Tissues exposed to ICG showed damage in every retinal layer and significantly higher release of LDH.
CONCLUSION
Exposure to ICG caused retinal damage in isolated rat retina tissue in our experimental model (in vitro).
目的
我们研究了与人工脑脊液（aCSF）相比，人工房水是否为合适的孵育介质，并使用在人工房水中孵育的离体大鼠视网膜，评估了两种活性染料——台盼蓝（TB）和吲哚菁绿（ICG）以及曲安奈德（TA）的视网膜毒性。
方法
在实验1中，分离视网膜节段并在aCSF、BSS plus、Opeguard Neo试剂盒或磷酸盐缓冲盐水（PBS）中孵育。在实验2中，将视网膜组织暴露于其中一种试剂，并在BSS plus中孵育。通过形态学检查和生化分析评估视网膜损伤，每小时测量一次培养基中的乳酸脱氢酶（LDH）。
结果
在实验1中，BSS plus被确认为合适的孵育介质。在实验2中，暴露于TB或TA的视网膜无明显变化。暴露于ICG的组织在每个视网膜层均显示损伤，且LDH释放显著更高。
结论
在我们的实验模型（体外）中，暴露于ICG会导致离体大鼠视网膜组织发生视网膜损伤。
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