Department of Ophthalmology, University Hospital, Dijon, France.
Retina. 2010 Sep;30(8):1294-301. doi: 10.1097/IAE.0b013e3181d205aa.
The purpose of this study was to evaluate the functional and structural damage of the retina after intravitreal injections of four different dyes in the rat.
Rats were injected intravitreally with indocyanine green (ICG), trypan blue, triamcinolone acetonide, or brilliant blue G in the right eye. The other eye was injected with saline and served as a control. Simultaneous bilateral electroretinograms were recorded before injection and 7 and 28 days after injection. Histology and immunohistochemistry analyses with antibodies recognizing glial fibrillary acidic protein and protein kinase C were performed 28 days after the initial injection on both eyes.
Seven days after dye injection, the electroretinogram response of the treated eyes was altered in each group. At 1 month, eyes injected with triamcinolone acetonide, trypan blue, or brilliant blue G fully recovered, whereas eyes treated with ICG had A-wave and B-wave reduction of 65% and 63%, respectively. The inner nuclear layer thickness was statistically decreased in the ICG group (P = 0.003) but not with other dyes. Protein kinase C staining was decreased in the ICG group only, but no abnormal qualitative staining was found with either glial fibrillary acidic protein or protein kinase C antibodies with any dye.
Among the four tested dyes, only ICG led to functional and structural retinal damage.
本研究旨在评估在大鼠中玻璃体内注射四种不同染料后视网膜的功能和结构损伤。
将吲哚菁绿（ICG）、台盼蓝、曲安奈德或亮蓝 G 注射到大鼠右眼玻璃体内。左眼注射生理盐水作为对照。在注射前和注射后 7 天和 28 天同时记录双侧视网膜电图。在初次注射后 28 天，对双眼进行组织学和免疫组织化学分析，使用识别神经胶质纤维酸性蛋白和蛋白激酶 C 的抗体。
在染料注射后 7 天，各组的视网膜电图反应均发生改变。1 个月时，接受曲安奈德、台盼蓝或亮蓝 G 治疗的眼睛完全恢复，而接受 ICG 治疗的眼睛 A 波和 B 波分别降低了 65%和 63%。ICG 组的内核层厚度在统计学上减少（P=0.003），但其他染料则没有。只有 ICG 组的蛋白激酶 C 染色减少，但任何染料均未发现神经胶质纤维酸性蛋白或蛋白激酶 C 抗体的异常定性染色。
在测试的四种染料中，只有 ICG 会导致视网膜的功能和结构损伤。
