Green Stefan J, Michel Frederick C, Hadar Yitzhak, Minz Dror
Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot, Israel.
FEMS Microbiol Lett. 2004 Apr 1;233(1):115-23. doi: 10.1016/j.femsle.2004.01.049.
We analyzed bacterial communities in two cow manure composts derived from the same feed manure and composted in the same location, but composted with different carbon amendments, and in peat-based potting mixes amended with these composts. Bacterial communities were characterized by PCR-denaturing gradient gel electrophoresis (DGGE) analysis of extracted DNAs, and population fingerprints generated for each sample were compared. Sequence analyses of dominant DGGE bands revealed that members of the phylum Bacteroidetes were the most dominant bacteria detected in this study (19 of 31 clones). These analyses demonstrate that bacterial community profiles of individual composts were highly similar, as were profiles of compost-amended potting mixes. However, potting mix profiles differed substantially from the original compost profiles and from that of the peat base. These data indicate that highly similar bacterial populations were active in the two composts, and suggest that the effects of the initial carbon amendment on the mature compost bacterial communities were minor, while factors such as the feed manure and composting location may have been more influential.
我们分析了源自相同饲料粪便且在同一地点堆肥，但添加了不同碳源改良剂的两种牛粪堆肥中的细菌群落，以及用这些堆肥改良的泥炭基盆栽混合料中的细菌群落。通过对提取的DNA进行PCR-变性梯度凝胶电泳（DGGE）分析来表征细菌群落，并比较每个样品生成的种群指纹图谱。对优势DGGE条带的序列分析表明，拟杆菌门成员是本研究中检测到的最优势细菌（31个克隆中有19个）。这些分析表明，各个堆肥的细菌群落概况高度相似，用堆肥改良的盆栽混合料的概况也是如此。然而，盆栽混合料的概况与原始堆肥概况以及泥炭基质的概况有很大不同。这些数据表明，两种堆肥中存在高度相似的活跃细菌种群，并表明初始碳源改良对成熟堆肥细菌群落的影响较小，而饲料粪便和堆肥地点等因素可能更具影响力。
