Crispi Stefania, Sanzari Emma, Monfregola Jlenia, De Felice Nicola, Fimiani Giorgia, Ambrosio Raffaele, D'Urso Michele, Ursini Matilde Valeria
Institute of Genetics and Biophysics Adriano Buzzati-Traverso, CNR, Via P. Castellino 111, 80131 Naples, Italy.
FEBS Lett. 2004 Mar 26;562(1-3):27-34. doi: 10.1016/S0014-5793(04)00166-8.
We recently published the genomic characterization of the STAT5A and STAT5B paralogous genes that are located head to head in the 17q21 chromosome and share large regions of sequence identity. We here demonstrate by transient in vitro transfection that STAT5A and STAT5B promoters are able to direct comparable levels of transcription. The expression of basal promoters is enhanced after Sp1 up-regulation in HeLa and SL2 cells while DNA methylation associated to the recruitment of MeCP2 methyl CpG binding protein down-regulates STAT5A and B promoters by interfering with Sp1-induced transcription. In addition, cross-species sequence comparison identified a bi-directional negative cis-acting regulatory element located in the STAT5 intergenic region.
我们最近发表了位于17q21染色体上且头对头排列并共享大片序列同一性区域的STAT5A和STAT5B旁系同源基因的基因组特征。我们在此通过瞬时体外转染证明，STAT5A和STAT5B启动子能够指导相当水平的转录。在HeLa和SL2细胞中Sp1上调后，基础启动子的表达增强，而与MeCP2甲基化CpG结合蛋白募集相关的DNA甲基化通过干扰Sp1诱导的转录来下调STAT5A和B启动子。此外，跨物种序列比较确定了位于STAT基因间区域的双向负性顺式作用调节元件。
