Song Ki-Ho, Ko Seung Hyun, Ahn Yu-Bae, Yoo Soon-Jib, Chin Hyung-Min, Kaneto Hideaki, Yoon Kun-Ho, Cha Bong-Yun, Lee Kwang-Woo, Son Ho-Young
Department of Internal Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Biochem Biophys Res Commun. 2004 Apr 16;316(4):1094-100. doi: 10.1016/j.bbrc.2004.02.153.
Despite a recent breakthrough in human islet transplantation for treating diabetes mellitus, the limited availability of insulin-producing tissue is still a major obstacle. Here, we studied whether adult pancreatic acinar cells have the potential to transdifferentiate into islet or beta cells. Pancreatic acini were isolated from 7- to 8-weeks-old male Sprague-Dawley rats and cultured in suspension. Within 1 week, most of the acinar cells lost amylase expression and converted to cells with a duct cell phenotype. Insulin-positive cells were also observed, mainly at the periphery of the acini-derived spheroids. Insulin gene and protein expression was increased. Presence of a few insulin-positive cells coexpressing cytokeratins suggests that a spontaneous acinar to ductal cell transdifferentiation process was further going on towards beta cells. This study provides the first evidence that adult pancreatic acinar cells could be differentiated into insulin-expressing cells in vitro.
尽管最近人类胰岛移植治疗糖尿病取得了突破，但胰岛素生成组织的有限可用性仍然是一个主要障碍。在这里，我们研究了成年胰腺腺泡细胞是否有潜力转分化为胰岛或β细胞。从7至8周龄的雄性Sprague-Dawley大鼠中分离出胰腺腺泡，并进行悬浮培养。在1周内，大多数腺泡细胞失去淀粉酶表达并转化为具有导管细胞表型的细胞。还观察到胰岛素阳性细胞，主要位于腺泡来源的球体周边。胰岛素基因和蛋白表达增加。少数共表达细胞角蛋白的胰岛素阳性细胞的存在表明，腺泡到导管细胞的自发转分化过程正在进一步朝着β细胞发展。这项研究提供了首个证据，证明成年胰腺腺泡细胞在体外可分化为胰岛素表达细胞。
