幽门螺杆菌在胃黏液中的空间取向。
The spatial orientation of Helicobacter pylori in the gastric mucus.
作者信息
Schreiber Sören, Konradt Manuela, Groll Claudia, Scheid Peter, Hanauer Guido, Werling Hans-Otto, Josenhans Christine, Suerbaum Sebastian
机构信息
Institut für Physiologie, Ruhr-Universität Bochum, D-44780 Bochum, Germany.
出版信息
Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):5024-9. doi: 10.1073/pnas.0308386101. Epub 2004 Mar 25.
Abstract
The highly motile human pathogen Helicobacter pylori lives deep in the gastric mucus layer. To identify which chemical gradient guides the bacteria within the mucus layer, combinations of luminal perfusion, dialysis, and ventilation were used to modify or invert transmucus gradients in anaesthetized Helicobacter-infected mice and Mongolian gerbils. Neither changes in lumen or arterial pH nor inversion of bicarbonate/CO2 or urea/ammonium gradients disturbed Helicobacter orientation. However, elimination of the mucus pH gradient by simultaneous reduction of arterial pH and bicarbonate concentration perturbed orientation, causing the bacteria to spread over the entire mucus layer. H. pylori thus uses the gastric mucus pH gradient for chemotactic orientation.
摘要
极具运动性的人类病原体幽门螺杆菌生活在胃黏液层深处。为了确定是哪种化学梯度引导细菌在黏液层中移动，研究人员采用管腔灌注、透析和通气相结合的方法，对麻醉的幽门螺杆菌感染小鼠和蒙古沙鼠的跨黏液梯度进行改变或反转。管腔或动脉pH值的变化，以及碳酸氢盐/二氧化碳或尿素/铵梯度的反转，均未干扰幽门螺杆菌的定向。然而，通过同时降低动脉pH值和碳酸氢盐浓度来消除黏液pH梯度，会扰乱细菌的定向，导致细菌扩散到整个黏液层。因此，幽门螺杆菌利用胃黏液pH梯度进行趋化定向。
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