炎性分泌型磷脂酶A2和胞质型磷脂酶A2在癌前和恶性Barrett食管中的表达

Expression of inflammatory secretory phospholipase A2 and cytosolic phospholipase A2 in premalignant and malignant Barrett's oesophagus.

作者信息

Lagorce-Pagès Christine, Paraf François, Wendum Dominique, Martin Antoine, Fléjou Jean-François

机构信息

Service d'Anatomie Pathologique, Hôpital Avicenne, AP-HP, Bobigny, France.

出版信息

Virchows Arch. 2004 May;444(5):426-35. doi: 10.1007/s00428-004-1003-7. Epub 2004 Mar 25.

DOI:10.1007/s00428-004-1003-7

Abstract

AIMS

To establish the prevalence of inflammatory secretory phospholipase A2 (sPLA2) and cytoplasmic phospholipase A2 (cPLA2) expression in a surgical series of Barrett's adenocarcinoma and associated preneoplastic lesions and to correlate this expression with clinicopathological data and prognosis.

METHODS

sPLA2 and cPLA2 were analysed by means of immunohistochemistry in surgical specimens of 67 and 73 cases of Barrett's adenocarcinomas, respectively. Barrett's mucosa was analysed in 31 cases.

RESULTS

Expression of sPLA2 was detected in 48% of Barrett's mucosa negative for intraepithelial neoplasia and 63% of Barrett's adenocarcinoma. Semi-quantitative analysis revealed a significant increase in sPLA2 expression between Barrett's mucosa negative for intraepithelial neoplasia and adenocarcinoma. cPLA2 expression was detected in 18% of Barrett's adenocarcinoma. An inverse correlation was found between cPLA2 expression and depth of tumour infiltration, neoplastic vascular invasion and neoplastic perineural invasion. Survival analysis showed no significant prognostic value for sPLA2 and cPLA2.

CONCLUSION

sPLA2 is frequently expressed in Barrett's oesophagus. The increasing expression of sPLA2 that we observed from Barrett's mucosa to adenocarcinoma suggests that sPLA2 could be involved in Barrett's carcinogenesis. In contrast, cPLA2 expression is less frequently observed in Barrett's oesophagus and is inversely associated with aggressive pathological features of the tumours.

摘要

目的

确定炎性分泌型磷脂酶A2（sPLA2）和胞质型磷脂酶A2（cPLA2）在一系列手术切除的巴雷特腺癌及相关癌前病变中的表达情况，并将该表达与临床病理数据及预后相关联。

方法

分别采用免疫组织化学方法对67例和73例巴雷特腺癌手术标本中的sPLA2和cPLA2进行分析。对31例巴雷特黏膜进行分析。

结果

在31例上皮内瘤变阴性的巴雷特黏膜中，48%检测到sPLA2表达；在巴雷特腺癌中，则有63%检测到sPLA2表达。半定量分析显示，上皮内瘤变阴性的巴雷特黏膜与腺癌之间，sPLA2表达显著增加。在18%的巴雷特腺癌中检测到cPLA2表达。发现cPLA2表达与肿瘤浸润深度、肿瘤血管侵犯及肿瘤神经周围侵犯呈负相关。生存分析显示，sPLA2和cPLA2无显著预后价值。

结论

sPLA2在巴雷特食管中常表达。我们观察到从巴雷特黏膜到腺癌sPLA2表达增加，提示sPLA2可能参与巴雷特癌变过程。相比之下，cPLA2在巴雷特食管中较少见，且与肿瘤侵袭性病理特征呈负相关。