在体内，细胞质动力蛋白促使微管成核，从而将它们组织成放射状阵列。
Cytoplasmic dynein nucleates microtubules to organize them into radial arrays in vivo.
作者信息
Malikov Viacheslav, Kashina Anna, Rodionov Vladimir
机构信息
Department of Physiology and Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, Connecticut 06032-1507, USA.
出版信息
Mol Biol Cell. 2004 Jun;15(6):2742-9. doi: 10.1091/mbc.e03-10-0770. Epub 2004 Mar 26.
Abstract
Numerous evidence demonstrates that dynein is crucial for organization of microtubules (MTs) into radial arrays, but its exact function in this process is unclear. Here, we studied the role of cytoplasmic dynein in MT radial array formation in the absence of the centrosome. We found that dynein is a potent MT nucleator in vitro and that stimulation of dynein activity in cytoplasmic fragments of melanophores induces nucleation-dependent formation of MT radial array in the absence of the centrosome. This new property of dynein, in combination with its known role as an MT motor that is essential for MT array organization in the absence and presence of the centrosome, makes it a unique molecule whose activity is necessary and sufficient for the formation and maintenance of MT radial arrays in cells.
摘要
大量证据表明，动力蛋白对于将微管（MTs）组织成放射状阵列至关重要，但其在这一过程中的具体功能尚不清楚。在此，我们研究了在没有中心体的情况下，细胞质动力蛋白在MT放射状阵列形成中的作用。我们发现，动力蛋白在体外是一种强大的MT成核剂，并且在黑素细胞的细胞质片段中刺激动力蛋白活性会在没有中心体的情况下诱导依赖成核的MT放射状阵列形成。动力蛋白的这一新特性，与其作为MT马达的已知作用相结合，而MT马达在有或没有中心体的情况下对于MT阵列组织都是必不可少的，这使其成为一种独特的分子，其活性对于细胞中MT放射状阵列的形成和维持是必要且充分的。
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Curr Biol. 2003 Oct 28;13(21):1837-47. doi: 10.1016/j.cub.2003.10.027.
2
Centrosome positioning in interphase cells.间期细胞中的中心体定位
J Cell Biol. 2003 Sep 15;162(6):963-9. doi: 10.1083/jcb.200305082.
3
Distinct cell cycle-dependent roles for dynactin and dynein at centrosomes.动力蛋白激活蛋白和动力蛋白在中心体上不同的细胞周期依赖性作用。
J Cell Biol. 2002 Oct 28;159(2):245-54. doi: 10.1083/jcb.200203089. Epub 2002 Oct 21.
4
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5
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6
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