Wang G Y, Hirai K, Shimada H, Taji S, Zhong S Z
Department of Anatomy, Kanazawa Medical University, Ishikawa, Japan.
Brain Res. 1992 Jun 26;583(1-2):216-26. doi: 10.1016/s0006-8993(10)80027-7.
Wistar rats (close cloned strain) were used to investigate the effect of endogenous laminin and fibronectin on axons, Schwann cells and perineurial cells in the regenerating peripheral nervous system (PNS). Sciatic nerve grafts obtained from donor rats were frozen, thawed and treated with rabbit anti-rat laminin or anti-fibronectin antiserum. Control grafts were treated with normal rabbit serum alone. One cm long portions of the sciatic nerve of the recipient rats were replaced with grafts. At 15 days after transplantation the number of regenerated axons in the laminin- and fibronectin-depleted grafts was half of that in the control. The growing axons in the laminin-depleted grafts did not recognize the basal lamina scaffolds (BLS) remaining in the basal lamina tubes, while in the control and fibronectin-depleted grafts 90% or more of axons grew inside the BLS. Elongation of axons always preceded migration of Schwann cells with the latter subsequently adhering to and wrapping around the former. Perineurium-forming fibroblastic cells recognized the combination of axons and Schwann cells and formed perineurial fasciculi around them. These fibroblastic cells did not recognize empty BLS but responded to them only when fibronectin was depleted. Macrophages sometimes closely faced the naked axons which elongated outside the BLS. These results suggest that in the early stages of nerve regeneration endogenous laminin and fibronectin not only regulate the growth of regenerating nerve fibers, but also exert a positive influence on perineurial cells and macrophages, both of which play important roles in nerve tissue injury and repair.
使用Wistar大鼠（近交克隆品系）来研究内源性层粘连蛋白和纤连蛋白对再生周围神经系统（PNS）中轴突、施万细胞和神经束膜细胞的影响。从供体大鼠获取的坐骨神经移植物经冷冻、解冻后，用兔抗大鼠层粘连蛋白或抗纤连蛋白抗血清处理。对照移植物仅用正常兔血清处理。将受体大鼠坐骨神经1厘米长的部分用移植物替换。移植后15天，层粘连蛋白和纤连蛋白缺失的移植物中再生轴突的数量是对照移植物中的一半。层粘连蛋白缺失的移植物中生长的轴突无法识别基底膜管中残留的基底膜支架（BLS），而在对照移植物和纤连蛋白缺失的移植物中，90%或更多的轴突在BLS内生长。轴突的伸长总是先于施万细胞的迁移，随后施万细胞附着并包裹轴突。形成神经束膜的成纤维细胞识别轴突和施万细胞的组合，并在它们周围形成神经束膜束。这些成纤维细胞不识别空的BLS，仅在纤连蛋白缺失时对其做出反应。巨噬细胞有时紧密靠近在BLS外伸长的裸露轴突。这些结果表明，在神经再生的早期阶段，内源性层粘连蛋白和纤连蛋白不仅调节再生神经纤维的生长，而且对神经束膜细胞和巨噬细胞产生积极影响，这两者在神经组织损伤和修复中都发挥着重要作用。
