利用极谱催化电流直接测定铁蛋白中的铁。
Direct determination of iron in ferritin using the polarographic catalytic current.
作者信息
Qiu J, Song J, Gao H
机构信息
Institute of Electroanalytical Chemistry, Northwest University, 710069, Xian, People's Republic of China.
出版信息
Anal Bioanal Chem. 1996 Aug;356(1):101-3. doi: 10.1007/s0021663560101.
A new method for the determination of iron in ferritin using the polarographic catalytic current has been developed. Iron is released in NaOH and triethanolamine (TEA) solution and subsequently determined based on the polarographic catalytic wave in the presence of bromate with the peak potential of -1.0 V (vs.SCE). The 2.5-derivative peak height of iron is linearly proportional to its concentration in the range of 8.0 x 10(-8)-1.0 x 10(-6) g/ml with a correlation coefficient of 0.9991. The method has been applied to the determination of iron in human fetal liver and horse-spleen ferritin and the recovery is 95-112%.
开发了一种使用极谱催化电流测定铁蛋白中铁的新方法。铁在氢氧化钠和三乙醇胺（TEA）溶液中释放，随后在溴酸盐存在下基于极谱催化波进行测定，其峰电位为-1.0 V（相对于饱和甘汞电极）。铁的2.5次导数峰高在8.0×10⁻⁸ - 1.0×10⁻⁶ g/ml范围内与其浓度呈线性比例关系，相关系数为0.9991。该方法已应用于测定人胎儿肝脏和马脾铁蛋白中的铁，回收率为95 - 112%。
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