Joung Y H, Youm J W, Jeon J H, Lee B C, Ryu C J, Hong H J, Kim H C, Joung H, Kim H S
Plant Genomics Laboratory, Korea Research Institute of Bioscience and Biotechnology, Yusung-Gu, P.O. Box 115, Taejeon, South Korea.
Plant Cell Rep. 2004 Jul;22(12):925-30. doi: 10.1007/s00299-004-0775-1. Epub 2004 Mar 27.
In an attempt to develop an edible vaccine, we transformed a recombinant hepatitis B virus (HBV) gene encoding the middle protein of HBV that contains the surface S and preS2 antigen into potato by Agrobacterium-mediated transformation. The HBV gene was under control of either the CaMV 35S promoter, the double 35S promoter with the AlMV 5' non-translated leader sequence, or the tuber-specific patatin promoter. HBV mRNA levels were higher with the 35S promoter than with the double 35S and patatin promoters; however, the levels of the S and preS2 antigen in the transformed tubers were higher with the patatin promoter than with the CaMV 35S and double promoters. The levels of preS2 antigen produced are the highest reported to date. Transgenic potato tubers were fed to mice, and the mice showed an immune response against the HBV S antigen.
为了研发一种可食用疫苗，我们通过农杆菌介导的转化方法，将编码乙肝病毒（HBV）中蛋白（包含表面S抗原和前S2抗原）的重组乙肝病毒基因导入马铃薯。乙肝病毒基因受花椰菜花叶病毒（CaMV）35S启动子、带有苜蓿花叶病毒（AlMV）5'非翻译前导序列的双35S启动子或块茎特异性patatin启动子的控制。使用35S启动子时乙肝病毒mRNA水平高于双35S启动子和patatin启动子；然而，使用patatin启动子时转化块茎中S抗原和前S2抗原的水平高于CaMV 35S启动子和双启动子。所产生的前S2抗原水平是迄今为止报道的最高水平。将转基因马铃薯块茎喂给小鼠，小鼠表现出针对乙肝病毒S抗原的免疫反应。
