Arakawa T, Chong D K, Langridge W H
Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, CA 92350, USA.
Nat Biotechnol. 1998 Mar;16(3):292-7. doi: 10.1038/nbt0398-292.
Transgenic potatoes were engineered to synthesize a cholera toxin B subunit (CTB) pentamer with affinity for GMI-ganglioside. Both serum and intestinal CTB-specific antibodies were induced in orally immunized mice. Mucosal antibody titers declined gradually after the last immunization but were restored following an oral booster of transgenic potato. The cytopathic effect of cholera holotoxin (CT) on Vero cells was neutralized by serum from mice immunized with transgenic potato tissues. Following intraileal injection with CT, the plant-immunized mice showed up to a 60% reduction in diarrheal fluid accumulation in the small intestine. Protection against CT was based on inhibition of enterotoxin binding to the cell-surface receptor GMI-ganglioside. These results demonstrate the ability of transgenic food plants to generate protective immunity in mice against a bacterial enterotoxin.
转基因土豆经过基因工程改造，可合成对GM1神经节苷脂具有亲和力的霍乱毒素B亚基（CTB）五聚体。口服免疫小鼠后，血清和肠道中均诱导产生了CTB特异性抗体。末次免疫后，黏膜抗体滴度逐渐下降，但在口服转基因土豆加强免疫后得以恢复。用转基因土豆组织免疫的小鼠血清可中和霍乱全毒素（CT）对Vero细胞的细胞病变效应。回肠内注射CT后，经植物免疫的小鼠小肠腹泻液积聚减少高达60%。对CT的保护作用基于抑制肠毒素与细胞表面受体GM1神经节苷脂的结合。这些结果证明了转基因食用植物在小鼠中产生针对细菌性肠毒素保护性免疫的能力。
