Dykman L A, Sumaroka M V, Staroverov S A, Zaĭtseva I S, Bogatyrev V A
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410049 Russia.
Izv Akad Nauk Ser Biol. 2004 Jan-Feb(1):86-91.
We studied the capacity of colloidal gold for enhancing specific and nonspecific immune response in laboratory animals (rabbits, rats, and mice) immunized with antigens of various nature. The antibody titers obtained with colloidal gold as a carrier were higher as compared to the standard immunization techniques (free antigen or Freund's adjuvant). Application of colloidal gold increased nonspecific immune responses as well: lysozyme concentration in the blood, activity of the complement system proteins, as well as phagocytic and bactericidal activities. The obtained antibodies were tested by immunodot assay using gold markers. Immunization of the animals with colloidal gold conjugates with haptens as well as complete antigens was shown to induce formation of highly active antibodies without using other antigens such as complete Freund's adjuvant. In addition, antigen quantities for animal immunization with colloidal gold was by one order of magnitude lower as compared to the complete Freund's adjuvant immunization. This fact can point to direct adjuvant activity of colloidal gold.
我们研究了胶体金增强用各种性质抗原免疫的实验动物（兔、大鼠和小鼠）特异性和非特异性免疫反应的能力。与标准免疫技术（游离抗原或弗氏佐剂）相比，以胶体金为载体获得的抗体滴度更高。胶体金的应用也增强了非特异性免疫反应：血液中溶菌酶浓度、补体系统蛋白活性以及吞噬和杀菌活性。使用金标记物通过免疫斑点试验对获得的抗体进行检测。结果表明，用胶体金与半抗原以及完全抗原的缀合物免疫动物，无需使用其他抗原如完全弗氏佐剂即可诱导形成高活性抗体。此外，与完全弗氏佐剂免疫相比，用胶体金免疫动物所需的抗原量低一个数量级。这一事实可能表明胶体金具有直接的佐剂活性。
