Mizuno K, Clark A F, Streilein J W
Department of Microbiology, University of Miami School of Medicine, FL 33101.
Invest Ophthalmol Vis Sci. 1989 Jun;30(6):1112-9.
Immune responses to cellular antigens placed in the anterior chamber of the eye are deviant: antibodies and cytotoxic T cells are generated, but delayed hypersensitivity is impaired. To determine whether a similar pattern of unusual reactivity would be induced by soluble antigens placed in this privileged site, we have examined the systemic immune responses of mice to anterior chamber injections of bovine serum albumin and bovine retinal S antigen--both soluble molecules. Recipients of intraocular injections of these antigens without adjuvant developed no detectable systemic immune response. When BSA was mixed with complete or incomplete Freund's adjuvant and injected into the anterior chamber, recipients produced serum specific antibodies; however, they displayed impaired delayed hypersensitivity. Anterior chamber recipients of soluble antigens subsequently proved refractory to the development of delayed hypersensitivity when immunogenic doses of the same antigens were placed subcutaneously. Moreover, the inability to mount delayed hypersensitivity could be adoptively transferred with spleen cells from animals that had previously received intraocular injections of bovine albumin or S antigen. It is concluded that soluble antigens, as well as surface membrane-bound antigens, are capable of inducing anterior chamber-associated immune deviation (ACAID). The possibility is discussed that the capacity of soluble retinal S antigen to induce ACAID may be pertinent to the maintenance of self-tolerance to this autologous, intraocular molecule.
会产生抗体和细胞毒性T细胞，但迟发型超敏反应受损。为了确定置于这个免疫赦免部位的可溶性抗原是否会诱导类似的异常反应模式，我们检测了小鼠对前房注射牛血清白蛋白和牛视网膜S抗原（两者均为可溶性分子）的全身免疫反应。无佐剂眼内注射这些抗原的受体未产生可检测到的全身免疫反应。当牛血清白蛋白与完全或不完全弗氏佐剂混合并注入前房时，受体产生了血清特异性抗体；然而，它们表现出迟发型超敏反应受损。当皮下注射免疫原性剂量的相同抗原时，可溶性抗原的前房受体随后被证明对迟发型超敏反应的发展具有抗性。此外，不能产生迟发型超敏反应可以通过先前接受眼内注射牛白蛋白或S抗原的动物的脾细胞进行过继转移。得出的结论是，可溶性抗原以及表面膜结合抗原都能够诱导前房相关免疫偏离（ACAID）。讨论了可溶性视网膜S抗原诱导ACAID的能力可能与维持对这种自体眼内分子的自身耐受性有关的可能性。
